原创力文档reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos重组rhesus-bovine转录组的种间体细胞核移植胚胎.pdfVIP
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reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos重组rhesus-bovine转录组的种间体细胞核移植胚胎
Reprogrammed Transcriptome in Rhesus-Bovine
Interspecies Somatic Cell Nuclear Transfer Embryos
1 2,3 1 4 4 1,5
Kai Wang , Hasan H. Otu , Ying Chen , Young Lee , Keith Latham , Jose B. Cibelli *
1 Michigan State University, East Lansing, Michigan, United States of America, 2 BIDMC Genomics Center, Harvard Medical School, Boston, Massachusetts, United States of
America, 3 Department of Bioengineering, Istanbul Bilgi University, Istanbul, Turkey, 4 Temple University, Philadelphia, Pennsylvania, United States of America, 5 Programa
Andaluz de Terapia Celular, Andalucia, Spain
Abstract
Background: Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo
development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive.
Methodology/Principal Findings: In this study, we analyzed the EGA-related transcriptome of rhesus-bovine iSCNT 8- to 16-
cell embryos and dissected the reprogramming process in terms of embryonic gene activation, somatic gene silencing, and
maternal RNA degradation. Compared with fibroblast donor cells, two thousand and seven genes were activated in iSCNT
embryos, one quarter of them reaching expression levels comparable to those found in in vitro fertilized (IVF) rhesus
embryos. This suggested that EGA in iSCNT embryos had partially recapitulated rhesus embryonic development. Eight
hundred and sixty somatic genes were not silenced properly and continued to be expressed in iSCNT embryos, which
indicated incomplete nuclear reprogramming. We compared maternal RNA degradation in bovine oocytes between bovine-
bovine SCNT and iSCNT embryos. While maternal RNA degradat
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