regulation of the dna damage response and gene expression by the dot1l histone methyltransferase and the 53bp1 tumour suppressordna损伤反应和基因表达的调控dot1l组蛋白甲基转移酶和53 bp1的肿瘤抑制基因.pdfVIP

Regulation of the DNA Damage Response and Gene Expression by the Dot1L Histone Methyltransferase and the 53Bp1 Tumour Suppressor 1 1 3 2 3 Jennifer FitzGerald , Sylvie Moureau , Paul Drogaris , Enda O’Connell , Nebiyu Abshiru , Alain 3,4 3,5 1 1 Verreault , Pierre Thibault , Muriel Grenon *, Noel F. Lowndes * 1 Genome Stability Laboratory, School of Natural Sciences, Centre for Chromosome Biology, National University of Ireland Galway, Galway, Ireland, 2 National Centre for ´ ´ Biomedical Engineering Sciences, National University of Ireland Galway, Galway, Ireland, 3 Institute for Research in Immunology and Cancer, Universite de Montreal, ´ ´ ´ ´ ´ ´ ´ ´ ´ Montreal, Quebec, Canada, 4 Departement de Pathologie et Biologie Cellulaire, Universite de Montreal, Montreal, Quebec, Canada, 5 Departement de Chimie, Universite ´ ´ ´ de Montreal, Montreal, Quebec, Canada Abstract Background: Dot1L, a histone methyltransferase that targets histone H3 lysine 79 (H3K79), has been implicated in gene regulation and the DNA damage response although its functions in these processes remain poorly defined. Methodology/Principal Findings: Using the chicken DT40 model system, we generated cells in which the Dot1L gene is disrupted to examine the function and focal recruitment of the 53Bp1 DNA damage response protein. Det