regulation of translation in haloarchaea 5′- and 3′-utrs are essential and have to functionally interact in vivo监管的翻译haloarchaea 5u2032和3u2032-utrs至关重要,必须功能体内进行交互.pdfVIP

Regulation of Translation in Haloarchaea: 59- and 39-UTRs Are Essential and Have to Functionally Interact In Vivo ¨ Mariam Brenneis, Jorg Soppa* Goethe-University, Institute for Molecular Biosciences, Frankfurt, Germany Abstract Recently a first genome-wide analysis of translational regulation using prokaryotic species had been performed which revealed that regulation of translational efficiency plays an important role in haloarchaea. In fact, the fractions of genes under differential growth phase-dependent translational control in the two species Halobacterium salinarum and Haloferax volcanii were as high as in eukaryotes. However, nothing is known about the mechanisms of translational regulation in archaea. Therefore, two genes exhibiting opposing directions of regulation were selected to unravel the importance of untranslated regions (UTRs) for differential translational control in vivo. Differential translational regulation in exponentially growing versus stationary phase cells was studied by comparing translational efficiencies using a reporter gene system. Translational regulation was not observed when 59-UTRs or 39-UTRs alone were fused to the reporter gene. However, their simultaneous presence was sufficient to transfer differential translational control from the native transcript to the reporter transcript. This was true for both directions of translational control. Translational regulation was completely abolished when stem loops in the 59-UTR were changed by mutagenesis. An ‘‘UTR-swap’’ experiment demonstrated that the direction of translational regulation is encoded in the 39-UTR, not in the 59-UTR. While much is known about 59-UTR- dependent translational control in bacteria, the reported findings provide the firs