requirement for a uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity要求uroplakin 3像蛋白质的发展斑马鱼前肾小管上皮细胞的功能,形态发生和极性.pdfVIP

Requirement for a Uroplakin 3a-Like Protein in the Development of Zebrafish Pronephric Tubule Epithelial Cell Function, Morphogenesis, and Polarity 1 1 1 2 3 Shalini Mitra , Stefan Lukianov , Wily G. Ruiz , Chiara Cianciolo Cosentino , Subramaniam Sanker , 3 2 1,3 Linton M. Traub , Neil A. Hukriede , Gerard Apodaca * 1 Department of Medicine Renal-Electrolyte Division, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, 2 Department of Developmental Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, 3 Department of Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America Abstract Uroplakin (UP)3a is critical for urinary tract development and function; however, its role in these processes is unknown. We examined the function of the UP3a-like protein Upk3l, which was expressed at the apical surfaces of the epithelial cells that line the pronephric tubules (PTs) of the zebrafish pronephros. Embryos treated with upk3l-targeted morpholinos showed decreased pronephros function, which was attributed to defects in PT epithelial cell morphogenesis and polarization including: loss of an apical brush border and associated phospho-ERM proteins, apical redistribution of the basolateral Na+/ K+–ATPase, and altered or diminished expression of the apical polarity complex proteins Prkcz (atypical protein kinase C zeta) and Pard3 (Par3). Upk3l missing its C-terminal cytoplasmic domain or containing mutations in conserved tyrosine or proline residues did not rescue, or only partially rescued the effects of Upk3l dep