rotavirus viroplasm fusion and perinuclear localization are dynamic processes requiring stabilized microtubules轮状病毒viroplasm融合和细胞核周围的本地化需要稳定微管的动态过程.pdfVIP

Rotavirus Viroplasm Fusion and Perinuclear Localization Are Dynamic Processes Requiring Stabilized Microtubules 1 3,4 1 1,2 Catherine Eichwald *, Francesca Arnoldi , Andrea S. Laimbacher , Elisabeth M. Schraner , 1 1,2 3 1 Cornel Fraefel , Peter Wild , Oscar R. Burrone , Mathias Ackermann 1 Institute of Virology, University of Zurich, Zurich, Switzerland, 2 Institute of Veterinary Anatomy, University of Zurich, Zurich, Switzerland, 3 International Centre for ` Genetic Engineering and Biotechnology, Trieste, Italy, 4 Dipartimento Clinico di Scienze Mediche, Chirurgiche e della Salute, Universita degli Studi di Trieste, Trieste, Italy Abstract Rotavirus viroplasms are cytosolic, electron-dense inclusions corresponding to the viral machinery of replication responsible for viral template transcription, dsRNA genome segments replication and assembly of new viral cores. We have previously observed that, over time, those viroplasms increase in size and decrease in number. Therefore, we hypothesized that this process was dependent on the cellular microtubular network and its associated dynamic components. Here, we present evidence demonstrating that viroplasms are dynamic structures, which, in the course of an ongoing infection, move towards the perinuclear region of the cell, where they fuse among each other, thereby gaining considerably in size and, simultaneouly, explaining the decrease in numbers. On the viral side, this process seems to depend o