rmrp is a non-coding rna essential for early murine developmentrmrp是一个非编码rna对小鼠早期发育至关重要.pdfVIP

RMRP Is a Non-Coding RNA Essential for Early Murine Development 1,2 1,2 1 1 3 Joseph Rosenbluh , Deepak Nijhawan , Zhao Chen , Kwok-Kin Wong , Kenkichi Masutomi *, William C. Hahn1,2* 1 Department of Medical Oncology, Dana–Farber Cancer Institute and Departments of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts, United States of America, 2 Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, 3 Division of Cancer Stem Cell, National Cancer Center Research Institute, Tokyo, Japan Abstract RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional). We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele. Furthermore, we were unable to obtain viable homozygous RMRP null mice, indicating that RMRP is essential for early embryonic development. Citation: Rosenbluh J, Nijhawan D, Chen Z, Wong K-K, Masutomi K, et al. (2011) RMRP Is a Non-Coding RNA Essential for Early Murine Development. PLoS ONE 6(10): e26270. doi:10.1371/journal.pone.0026270 Editor: Saverio Bellusci, Childrens Hospital Los Angeles, United States of America Received June 10, 2011; Accepted September 23, 2011; Published October 19, 2011 Copyright: 2011 Rosenbluh et al. This is an open-access articl