schistosoma japonicum soluble egg antigens attenuate ifn-γ-induced mhc class ii expression in raw 264.7 macrophages日本血吸虫抗原减弱ifn-γ-induced可溶性鸡蛋mhc ii级表达原始264.7巨噬细胞.pdfVIP

Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-c-Induced MHC Class II Expression in RAW 264.7 Macrophages 1 ¤ 4 1 5 1,2 1,2,3 1,2 Gui-Xia Tang * , He-Jun Zhou , Jin-Wei Xu , Jin-Mei Xu , Min-Jun Ji , Hai-Wei Wu , Guan-Ling Wu * 1 Department of Pathogen Biology and Immunology, Nanjing Medical University, Nanjing, Jiangsu, China, 2 Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China, 3 Center for International Health Research, Rhode Island Hospital, Providence, Rhode Island, United States of America, 4 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, MOH, China, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai, China, 5 MacroStat Clinical Research Co. Ltd, Shanghai, China Abstract Innate immune response plays the key role in initiating and guiding the immune response. Elucidating the innate immune related molecular events involved in the interaction between the parasite and the host will aid in the development of an effective vaccine and anti-schistosome pharmaceuticals. In this study, we examined the regulatory effect of Schistosoma japonicum soluble egg antigen (SEA) on MHC class II expression in macrophage cell line RAW 264.7. We demonstrated that SEA possesses the ability to down-regulate IFN-c-induced MHC class II expression in RAW 264.7 cells. The production of IL- 10 and IL-6 in RAW 264.7 cells, induced by SEA, was responsible for mediating the down-regulation of MHC class II. Our findings suggest that in RAW 264.7 cells (1) IFN-c provides a condition for lower concentrations of SEA to attenuate MHC class II expression;