schistosoma japonicum udp-glucose 4-epimerase protein is located on the tegument and induces moderate protection against challenge infection日本血吸虫udp-glucose 4-epimerase蛋白质位于外皮和诱发感染适度保护的挑战.pdfVIP

Schistosoma Japonicum UDP-Glucose 4-Epimerase Protein Is Located on the Tegument and Induces Moderate Protection against Challenge Infection 1 1,2 1 1 1 1 1 1 Pingping Liu , Yanli Shi , Yunxia Yang , Yufan Cao , Yaojun Shi , Hao Li , Jinming Liu , Jiaojiao Lin , Yamei Jin1* 1 National Laboratory of Animal Schistosomiasis Control/Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, People’s Republic of China, 2 College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang Province, People’s Republic of China Abstract Schistosomiasis is an important global public health problem, as millions of people are at risk of acquiring this infection. An ideal method for sustainable control of schistosomiasis is using a vaccine alone or in combination with drugs. In the present study, we cloned the SjGALE gene and generated the expression product in E. coli. The expression level of SjGALE during different developmental stages of S. japonicum was evaluated by real-time RT-PCR and western blotting. Immunolocal- ization indicated that the protein was mainly located on the tegument of the parasite. Infection of rSjGALE-immunized mice demonstrated a 34% and 49% reduction of the mean worm burden and liver egg burden, respectively, in two independent experiments, indicating immune protection. The liver egg count from each female adult worm was significantly reduced by 63% in the two trials. The cytokine profile and IgG isotype analysis demonstrated the induction of a Th1 immune profile in response to immunization with this protein, further suggesting protection against infection. In conclusion, these f