原创力文档selecting temperature for protein crystallization screens using the temperature dependence of the second virial coefficient选择温度对蛋白质结晶的屏幕使用第二维里系数对温度的依赖关系.pdfVIP
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selecting temperature for protein crystallization screens using the temperature dependence of the second virial coefficient选择温度对蛋白质结晶的屏幕使用第二维里系数对温度的依赖关系
Selecting Temperature for Protein Crystallization Screens
Using the Temperature Dependence of the Second Virial
Coefficient
Jun Liu, Da-Chuan Yin*, Yun-Zhu Guo, Xi-Kai Wang, Si-Xiao Xie, Qin-Qin Lu, Yong-Ming Liu
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi, People’s Republic of China
Abstract
Protein crystals usually grow at a preferable temperature which is however not known for a new protein. This paper reports
a new approach for determination of favorable crystallization temperature, which can be adopted to facilitate the
crystallization screening process. By taking advantage of the correlation between the temperature dependence of the
second virial coefficient (B22) and the solubility of protein, we measured the temperature dependence of B22 to predict the
temperature dependence of the solubility. Using information about solubility versus temperature, a preferred crystallization
temperature can be proposed. If B22 is a positive function of the temperature, a lower crystallization temperature is
recommended; if B22 shows opposite behavior with respect to the temperature, a higher crystallization temperature is
preferred. Otherwise, any temperature in the tested range can be used.
Citation: Liu J, Yin D-C, Guo Y-Z, Wang X-K, Xie S-X, et al. (2011) Selecting Temperature for Protein Crystallization Screens Using the Temperature Dependence of
the Second Virial Coefficient. PLoS ONE 6(3): e17950. doi:10.1371/journal.pone.0017950
Editor: Vladimir Uversky, University of South Florida College of Medicine, United States of America
Received December 30, 2010; Accepted February 16, 2011; Published March 30, 2011
Copyright: 2011 Liu et al. This is an open-access article distributed under the terms of the Creat
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