sequence analysis of pkf3-70 in klebsiella pneumoniae probable origin from r100-like plasmid of escherichia coli序列分析pkf3 - 70的肺炎克雷伯菌可能起源r100-like大肠杆菌的质粒.pdfVIP

Sequence Analysis of pKF3-70 in Klebsiella pneumoniae: Probable Origin from R100-Like Plasmid of Escherichia coli 1,2. 1. 1 1 1 1 1 1 Huiguang Yi , Yali Xi , Jing Liu , Junrong Wang , Jinyu Wu , Teng Xu , Wei Chen , Biaobang Chen , 1 1 1 1 1 3 1 Meili Lin , Huan Wang , Mingming Zhou , Jinsong Li , Zuyuan Xu , Shouguang Jin , Qiyu Bao * 1 Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical College, Wenzhou, China, 2 T-Life Research Center, Fudan University, Shanghai, China, 3 Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, United States of America Abstract Background: Klebsiella pneumoniae is a clinically significant species of bacterium which causes a variety of diseases. Clinical treatment of this bacterial infection is greatly hindered by the emergence of multidrug-resistant strains. The resistance is largely due to the acquisition of plasmids carrying drug-resistant as well as pathogenic genes, and its conjugal transfer facilitates the spread of resistant phenotypes. Methodology/Principal Findings: The 70,057 bp plasmid pKF3-70, commonly found in Klebsiella pneumoniae, is composed of five main functional modules, including regions involved in replication, partition, conjugation, transfer leading, and variable regions. This plasmid is more similar to several Escherichia coli plasmids than any previously reported K. pneumoniae plasmids and pKF3-70 like plasmids share a common and conserved backbone sequence. The replication system of the pKF3-7