serratia marcescens is able to survive and proliferate in autophagic-like vacuoles inside non-phagocytic cells粘质沙雷氏菌能够生存和增殖在autophagic-like non-phagocytic细胞内液泡.pdfVIP

Serratia marcescens Is Able to Survive and Proliferate in Autophagic-Like Vacuoles inside Non-Phagocytic Cells 1. 2. 1. ´ 2 Griselda V. Fedrigo , Emanuel M. Campoy , Gisela Di Venanzio , Marıa Isabel Colombo , Eleonora ´ ´ 1* Garcıa Vescovi ´ ´ ´ ´ 1 Instituto de Biologıa Molecular y Celular de Rosario (IBR-CONICET), Departamento de Microbiologıa, Facultad de Ciencias Bioquımicas y Farmaceuticas, Universidad ´ ´ ´ ´ Nacional de Rosario, Rosario, Argentina, 2 Laboratorio de Biologıa Celular y Molecular-Instituto de Histologıa y Embriologıa (IHEM), Facultad de Ciencias Medicas, Universidad de Cuyo, CONICET, Mendoza, Argentina Abstract Serratia marcescens is an opportunistic human pathogen that represents a growing problem for public health, particularly in hospitalized or immunocompromised patients. However, little is known about factors and mechanisms that contribute to S. marcescens pathogenesis within its host. In this work, we explore the invasion process of this opportunistic pathogen to epithelial cells. We demonstrate that once internalized, Serratia is able not only to persist but also to multiply inside a large membrane-bound compartment. This structure displays autophagic-like features, acquiring LC3 and Rab7, markers described to be recruited throughout the progression of antibacterial