serratia odorifera a midgut inhabitant of aedes aegypti mosquito enhances its susceptibility to dengue-2 virus沙雷氏菌属odorifera埃及伊蚊的中肠居民增强了其dengue-2病毒的易感性.pdfVIP

Serratia odorifera a Midgut Inhabitant of Aedes aegypti Mosquito Enhances Its Susceptibility to Dengue-2 Virus 1 1 2 1,3 Anjali Apte-Deshpande , Mandar Paingankar , Mangesh D. Gokhale , Dileep N. Deobagkar * 1 Molecular Biology Research Laboratory, Department of Zoology, Center for Advance Studies, University of Pune, Pune, India, 2 Department of Medical Entomology and Zoology, National Institute of Virology, Pune, India, 3 Vice Chancellor, Goa University, Taleigaon Plateau, Goa, India Abstract Mosquito midgut plays a crucial role in its vector susceptibility and pathogen interaction. Identification of the sustainable microflora of the midgut environment can therefore help in evaluating its contribution in mosquito-pathogen interaction and in turn vector competence. To understand the bacterial diversity in the midgut of Aedes aegypti mosquitoes, we conducted a screening study of the gut microbes of these mosquitoes which were either collected from fields or reared in the laboratory ‘‘culture-dependent’’ approach. This work demonstrated that the microbial flora of larvae and adult Ae. aegypti midgut is complex and is dominated by Gram negative proteobacteria. Serratia odorifera was found to be stably associated in the midguts of field collected and laboratory reared larvae and adult females. The potential influence of this sustainable gut microbe on DENV-2 susceptibility of this vector was evaluated by co-feeding S. odorifera with DENV-2 to adult Ae. aegypti females (free of gut flora). The observations revealed that the viral susceptibility of these Aedes females enhanced significantly as compared to solely dengue-2 fed and anothe