sh2d2a modulates t cell mediated protection to a b cell derived tumor in transgenic micesh2d2a调节t细胞介导的保护转基因小鼠的b细胞衍生的肿瘤.pdfVIP

SH2D2A Modulates T Cell Mediated Protection to a B Cell Derived Tumor in Transgenic Mice 1,3 1 2 1 Tone Berge *, Ingrid Helene Bø Grønningsæter , Kristina Berg Lorvik , Greger Abrahamsen , 1 1 2 2 1 Stine Granum , Vibeke Sundvold-Gjerstad , Alexandre Corthay , Bjarne Bogen , Anne Spurkland 1 Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, Oslo, Norway, 2 Centre for Immune Regulation, Department of Immunology, Oslo ˚ University Hospital Rikshospitalet and University of Oslo, Oslo, Norway, 3 Department of Neurology, Oslo University Hospital Ulleval, Oslo, Norway Abstract Background: T cell specific adapter protein (TSAd), encoded by the SH2D2A gene, modulates signaling downstream of the T cell receptor (TCR). Young, unchallenged SH2D2A-deficient C57BL/6 mice exhibit a relatively normal immune phenotype. To address whether SH2D2A regulates physiologic immune responses, SH2D2A-deficient TCR-transgenic BALB/c mice were generated. The transgenic TCR recognizes a myeloma-derived idiotypic (Id) peptide in the context of the major histocompatibility complex (MHC) class II molecule I-Ed, and confers T cell mediated resistance to transplanted multiple myeloma development in vivo. Principal Findings: The immune phenotype of SH2D2A-deficient C57BL/6 and BALB/c mice did not reveal major differences compared to the corresponding wild type mice. When challenged with myelo