sgnp an essential stress granulenucleolar protein potentially involved in 5.8s rrna processingtransportsgnp必不可少的压力granulenucleolar蛋白可能参与5.8 s rrna processingtransport.pdfVIP

SGNP: An Essential Stress Granule/Nucleolar Protein Potentially Involved in 5.8s rRNA Processing/Transport Chun-Hong Zhu, Jinyong Kim, Jerry W. Shay, Woodring E. Wright* Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America Abstract Background: Stress Granules (SG) are sites of accumulation of stalled initiation complexes that are induced following a variety of cellular insults. In a genetic screen for factors involved in protecting human myoblasts from acute oxidative stress, we identified a gene encoding a protein we designate SGNP (Stress Granule and Nucleolar Protein). Methodology/Principal Findings: A gene-trap insertional mutagenesis screen produced one insertion that conferred resistance to sodium arsenite. RT-PCR/39 RACE was used to identify the endogenous gene expressed as a GFP-fusion transcript. SGNP is localized in both the cytoplasm and nucleolus and defines a non-nucleolar compartment containing 5.8S rRNA, a component of the 60S ribosomal subunit. Under oxidative stress, SGNP nucleolar localization decreases and it rapidly co-localizes with stress granules. The decrease in nucleolar SGNP following oxidative stress was accompanied by a large increase in nucleolar 5.8S rRNA. Knockdown of SGNP with shRNA increased global mRNA translation but induced growth arrest and cell death. Conclusions: These results suggest that SGNP is an essential gene that may be involved in ribosomal biogenesis and translational control in response to oxidative stress. Citation: Zhu C-H, Kim J, Shay JW, Wright WE (2008) SGNP: An Essential Stress Granule/Nucleolar Protein Potentially Involved in 5.8s rRNA Processing/ Transport. PLoS ONE 3(11): e3716. doi:10.1371/journal.pone.0003716 Edito