selective over-expression of endothelin-1 in endothelial cells exacerbates inner retinal edema and neuronal death in ischemic retina在内皮细胞的选择性表达endothelin-1加剧内心的视网膜水肿和视网膜缺血性神经元死亡.pdfVIP

Selective Over-Expression of Endothelin-1 in Endothelial Cells Exacerbates Inner Retinal Edema and Neuronal Death in Ischemic Retina 1 1 1 2,4 3,4 Simon S. F. Cheung , Justin W. C. Leung , Amy K. M. Lam , Karen S. L. Lam , Stephen S. M. Chung , Amy C. Y. Lo1,4¤*, Sookja K. Chung1,4* 1 Department of Anatomy, The University of Hong Kong, Hong Kong, China, 2 Department of Medicine, The University of Hong Kong, Hong Kong, China, 3 Department of Physiology, The University of Hong Kong, Hong Kong, China, 4 Research Centre of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China Abstract The level of endothelin-1 (ET-1), a potent vasoconstrictor, was associated with retinopathy under ischemia. The effects of endothelial endothelin-1 (ET-1) over-expression in a transgenic mouse model using Tie-1 promoter (TET-1 mice) on pathophysiological changes of retinal ischemia were investigated by intraluminal insertion of a microfilament up to middle cerebral artery (MCA) to transiently block the ophthalmic artery. Two-hour occlusion and twenty-two-hour reperfusion were performed in homozygous (Hm) TET-1 mice and their non-transgenic (NTg) littermates. Presence of pyknotic nuclei in ganglion cell layer (GCL) was investigated in paraffin sections of ipsilateral (ischemic) and contralateral (non-ischemic) retinae, followed by measurement of the thickness of inner retinal layer. Moreover, immunocytochemistry of glial fibrillary acidic protein (GFAP), glutamine synthetase (GS) and aquaporin-4 (AQP4) peptides on retinal sections were performed to study