signalling pathways involved in adult heart formation revealed by gene expression profiling in drosophila信号通路参与成人心脏形成了果蝇的基因表达分析.pdfVIP

Signalling Pathways Involved in Adult Heart Formation Revealed by Gene Expression Profiling in Drosophila 1,2 ´ ´ 1,2 ´ 3,4,5,6 3,4,5,6 ´ ´ 1,2 1,2* Bruno Zeitouni , Sebastien Senatore , Dany Severac , Cindy Aknin , Michel Semeriva , Laurent Perrin ´ ´ ´ ´ ´ 1 Institut de Biologie du Developpement de Marseille-Luminy, Universite de la Mediterranee, Marseille, France, 2 CNRS, UMR 6216, Marseille, France, 3 Institut de Genomique ´ Fonctionnelle, Montpellier, France, 4 CNRS, UMR 5203, Montpellier, France, 5 Universites Montpellier 1 et 2, Montpellier, France, 6 INSERM, U661, Montpellier, France Drosophila provides a powerful system for defining the complex genetic programs that drive organogenesis. Under control of the steroid hormone ecdysone, the adult heart in Drosophila forms during metamorphosis by a remodelling of the larval cardiac organ. Here, we evaluated the extent to which transcriptional signatures revealed by genomic approaches can provide new insights into the molecular pathways that underlie heart organogenesis. Whole-genome expression profiling at eight successive time-points covering adult heart formation revealed a highly dynamic temporal map of gene expression through 13 transcript clusters with distinct expression kinetics. A functional atlas of the transcriptome profile strikingly points to the genomic transcriptional response of the ecdysone cascade, and a sharp regulation of key components belonging to a few evolutionarily conserved signalling pa