sensitization prevalence, antibody cross-reactivity and immunogenic peptide profile of api g 2, the non-specific lipid transfer protein 1 of celery敏感的普遍性、抗体大和免疫原性肽的api g 2,芹菜的非特异性脂质转运蛋白1.pdfVIP

Sensitization Prevalence, Antibody Cross-Reactivity and Immunogenic Peptide Profile of Api g 2, the Non-Specific Lipid Transfer Protein 1 of Celery 1 1 1 2 1 Gabriele Gadermaier *, Michael Hauser , Matthias Egger , Rosetta Ferrara , Peter Briza , Keity Souza 3 2 1 4 2 1 Santos , Danila Zennaro , Tamara Girbl , Laurian Zuidmeer-Jongejan , Adriano Mari , Fatima Ferreira 1 Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, Salzburg, Austria, 2 Center for Molecular Allergology, IDI-IRCCS, Rome, Italy, 3 Discipline of Allergy and Immunology, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil, 4 Laboratory of Allergy, Department of Experimental Immunology, Academic Medical Center, Amsterdam, The Netherlands Abstract Background: Celery (Apium graveolens) represents a relevant allergen source that can elicit severe reactions in the adult population. To investigate the sensitization prevalence and cross-reactivity of Api g 2 from celery stalks in a Mediterranean population and in a mouse model. Methodology: 786 non-randomized subjects from Italy were screened for IgE reactivity to rApi g 2, rArt v 3 (mugwort pollen LTP) and nPru p 3 (peach LTP) using an allergen microarray. Clinical data of 32 selected patients with reactivity to LTP under investigation were evaluated. Specific IgE titers and cross-inhibitions were performed in ELISA and allergen microarray. Balb/ c mice were immunized with purified LTPs; IgG titers were determined in ELISA and mediator release was examined using RBL-2H3 cells. Simulated endolysosomal digestion was performed using microsomes obtained from hu