somaclonal variation is induced de novo via the tissue culture process a study quantifying mutated cells in saintpaulia体细胞无性系变异是通过组织培养诱导新创过程量化saintpaulia突变细胞的研究.pdfVIP

Somaclonal Variation Is Induced De Novo via the Tissue Culture Process: A Study Quantifying Mutated Cells in Saintpaulia Mitsuru Sato, Munetaka Hosokawa*, Motoaki Doi Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan Abstract Background: The origin of somaclonal variation has not been questioned previously, i.e., ‘‘pre-existing mutations’’ in explants and ‘‘newly induced mutations’’ arising from the tissue culture process have not been distinguished. This is primarily because there has been no reliable molecul