species delimitation in taxonomically difficult fungi the case of hymenogaster物种界定在分类学的困难真菌hymenogaster的情况.pdfVIP

Species Delimitation in Taxonomically Difficult Fungi: The Case of Hymenogaster 1 2 ´ 2 2 3 Benjamin Stielow , Zoltan Bratek , Akos Kund I Orczan , Szabolcs Rudnoy , Gunnar Hensel , Peter 1 1 ¨ 1 Hoffmann , Hans-Peter Klenk , Markus Goker * 1 DSMZ – German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany, 2 Department of Plant Physiology and Molecular Plant Biology, ELTE University, Budapest, Hungary, 3 Fungarium Gunnar Hensel, Merseburg, Germany Abstract Background: False truffles are ecologically important as mycorrhizal partners of trees and evolutionarily highly interesting as the result of a shift from epigeous mushroom-like to underground fruiting bodies. Since its first description by Vittadini in 1831, inappropriate species concepts in the highly diverse false truffle genus Hymenogaster has led to continued confusion, caused by a large variety of prevailing taxonomical opinions. Methodology: In this study, we reconsidered the species delimitations in Hymenogaster based on a comprehensive collection of Central European taxa comprising more than 140 fruiting bodies from 20 years of field work. The ITS rDNA sequence dataset was subjected to phylogenetic analysis as well as clustering optimization using OPTSIL software. Conclusions: Among distinct species concepts from the literature used to create reference partitions for clustering optimization, the broadest concept resulted in the highest agreement with the ITS data. Our results indicate a highly variable morphology of H. citrinus and H. griseus, most likely linked to environmental influences