strong association of 677 ct substitution in the mthfr gene with male infertility - a study on an indian population and a meta-analysis强大的协会677 ct替换mthfr基因与男性不育研究印度人口和荟萃分析.pdfVIP

Strong Association of 677 C.T Substitution in the MTHFR Gene with Male Infertility - A Study on an Indian Population and a Meta-Analysis 1 2 1 2 2 2 Nishi Gupta , Saraswati Gupta , Madhukar Dama , Archana David , Geeta Khanna , Anil Khanna , Singh Rajender1* 1 Division of Endocrinology, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, Uttar Pradesh, India, 2 IVF Department, Ajanta Hospitals and IVF Centre Pvt. Ltd., Lucknow, Uttar Pradesh, India Abstract Background: Methylenetetrahydrofolate reductase (MTHFR) is an important enzyme of folate and methionine metabolism, making it crucial for DNA synthesis and methylation. The objective of this study was to analyze MTHFR gene 677C.T polymorphism in infertile male individuals from North India, followed by a meta-analysis on our data and published studies. Methodology/Principal Findings: We undertook genotyping on a total of 837 individuals including well characterized infertile (N = 522) and confirmed fertile (N = 315) individuals. The SNP was typed by direct DNA sequencing. Chi square test was done for statistical analysis. Published studies were searched using appropriate keywords. Source of data collection for meta-analysis included ‘Pubmed’, ‘Ovid’ and ‘Google Scholar’. Those studies analyzing 677C.T polymorphism in male infertility and presenting all relevant data were included in meta-analysis. The genotype data for infertile subjects and fertile controls was extracted from each study. Chi square test was done to obtain odds ratio (OR) and p-value. Meta-analysis was performed using Comprehensive Meta-analysis software (Version 2). The frequency of mutant (T) allele (p = 0.0025) and