一种寄生茶小绿叶蝉蜡菌的鉴定及产孢条件优化.PDF

微生物学通报 May 20, 2016, 43(5): 935−941 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.150697 研究报告 一种寄生茶小绿叶蝉蜡蚧菌的鉴定及产孢条件优化 * 周叶鸣 邹晓 瞿娇娇 周家喜 李娟 ( 550025) 摘 要： 【目的】对寄生茶小绿叶蝉的丝状真菌进行鉴定和分生孢子培养研究。【方法】采用 形态特征比较和转录间隔区(ITS)序列构建系统树进行分析，分生孢子培养通过单因素筛选和正 交试验进行产孢条件优化。【结果】根据形态特征比较和系统发育分析表明，该真菌为渐狭蜡蚧 菌Lecanicillium attenuatum Zare W. Gams 。最优产孢条件(质量体积比)为：蛋白胨2% ，麦芽糖 1%，蚕蛹粉 1%，氯化钾 0.05% ，磷酸氢二钾 0.1% ，七水硫酸镁 0.05% ，琼脂 1.5%，蒸馏水 1 000 mL，25 °C 。【结论】通过形态特征比较和分子序列分析表明，罹病茶小绿叶蝉上的真菌 为已知种——渐狭蜡蚧菌，并对该菌株进行了产孢条件的优化。研究结果为该菌株应用到茶小绿 叶蝉的生物防治研究提供基础资料。 关键词：蜡蚧菌，渐狭蜡蚧菌，小绿叶蝉，产孢条件 Identification of a parasitic Lecanicillium of tea lesser leafhopper and optimization of sporulation conditions * ZHOU Ye-Ming ZOU Xiao QU Jiao-Jiao ZHOU Jia-Xi LI Juan (Institute of Fungal Resource, College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China) Abstract: [Objective] The objectives are to identify a parasitic Lecanicillium W. Gams Zare of tea lesser leafhopper and optimize sporulation culture condition of this fungus. [Methods] The comparison of morphological characteristics and internal transcribed spacer (ITS) sequence analysis were used for identification of the fungal species. Single factor test and orthogonal test were carried out to optimize sporulation conditions. [Results] According to the morphological characterization and phylogenetic tree, the fungus was identified as Lecanicillium attenuatum Zare W. Gams. The optimum condition of or s