CGRP对血管平滑肌细胞增殖及CDK2和Cyclin+E表达影响.pdfVIP

结论： CGRP 能抑制血清诱导大鼠VSMCs 的增殖，其机制可能是通过抑制细 胞ERK1/2 信号通路，下调细胞周期调节蛋白CDK2 和CyclinE 的表达， 阻滞细胞周期由G1 期进入S 期而发挥作用 关键词： 血管平滑肌细胞；CGRP ；增殖；p-ERK1/2 ；CDK2 ；CyclinE 6 Effect of CGRP on the proliferation of vascular smooth cell and expression of CDK2 and Cyclin E [ Abstract ] AIM To investigate the effect of calcitonin gene-related peptide(CGRP) on the proliferation of vascular smooth muscle cells induced by 10%FBS, and further explore whether this effect is related to the activity of ERK1/2 and the expression of CDK2 ,CyclinE. METHODS Vascular smooth muscle cells(VSMCs)were prepared from thoracic aortas of male Sprague-Dawley rat by the explant method. The passages of cells 3~10 were used for the present studies. The viability and cell cycle of cultured VSMCs were respectively estimated by MTT assay and Flow Cytometry (FCM).Western blot was used to observe the effects of pretreatment with CGRP on the expression of p-ERK1/2, CDK2, CyclinE induced by 10%FBS in VSMCs. RESULTS 10%FBS or Ang Ⅱ(100nM) time-dependently increased the viability of VSMCs. CGRP dose-dependently decreased the viability of VSMCs. Pretreatment of the cell with CGRP significantly inhibited VSMCs proliferation induced by 10%FBS. FCM a