俞募配穴对功能性消化不良模型大鼠血浆小分子代谢产物及胃泌素影响.pdf

成都中医药大学2007 级硕士论文 ABSTRACT Object: To study the influence of Shu-Mu compatibility on the alterations of slipoid metabolites in the blood plasma in FD rats by using 1H-NMR technique, and the influence of Shu-Mu compatibility on the gastrin in the blood plasma in FD rats by using ELISA. Methods: Fifty Wistar rats were randomly divided into five groups(n= 10): Control group,Model group,Wei shu (BL 21) group,Zhong wan (RN 12) group and Wei shu (BL 21) adds Zhong wan (RN 12) group .Acupoints Wei shu (BL 21) and Zhong wan (RN 12) were selected and treated with the stimulating power which kept local muscle of rats slightly shaken for 20min(thin-dense alternative wave, frequency 2- 100HZ, electric volt 2-4V), once a day, 10 days are in total. 1H-NMR technique was used to obtain the small molecules metabolic spectra of the blood plasma slipoid,the data were normalized, integrated and treated by SIMCA-P10.0 software pack to analize the major component (PCA) and the partial leastquares discriminate (PLS-DA) in combination with OSC method, in this way, the corresponding score scattering plot and loading plot were obtained. And ELISA technique was used to determine the content of gastrin in the blood plasma in FD rats. Results: 1. The result of assaying of gastrin: First, the interclass difference between the model group and the control group possess statistical significance; Second, the interclass differences among every acupuncture groups and the model group possess statistical significance; Third, the interclass differences among the Wei shu (BL 21) group, Zhong wan (RN 12) group and Wei shu (BL 21) adds Zhong wan (RN 12) group possess statistical significance. the result