银翘散对流感病毒感染小鼠模型肺组织mBDF1表达影响.pdf

成都中医药大学 ABSTRACT Objective To discuss the influnce of YinQiaoSan to β-defensin1 gene expression of model mouse lung tissue infectted by flu virus.Partly post the immunity charge mechanism of anti-virus by Chinese Medicine compound .Lend experiment basis for clinic application of YinQiaoSan preventing and cureing flu virus Methods （1）Influenza virus are Passage and determined its Virulence. Influenza H1N1 virus infection 9 day-old vaccinations allantoic cavity nestin chicken embryos, red blood clots measured for Virulence. （2 ） mice are infected by influenza virus. mice are infected with different concentrations of influenza virus When they are Anesthesiaed by aether. intranasal mice were LD50. Detecting their LD50 （3）mice divide into groups and treat. mice randomly divided into Chinese traditional medicine group, the western medicine group, the model group. In the ether 4LD50 infection in drugged, two days before the mice infected, TCM group to begin with YinQiaoSan scattered 60g/kg/d gastric lavage, Western medicine group to bingDuZuo 0.07 g/kg/d intraperitoneal injection, The model group releases physiological saline, d2, lavage d4 group, Chinese traditional medicine group and the group d8 western groups of mice were in the first 2 days after infection, 4 days, the first eight days of death, lung homogenate extraction using Real V 成都中医药大学 time - RNA polymerase chain reaction (PCR) detecting group RT - mouse lung tissue DEFB1 expression. Experimental results with standard deviation , followed by SPSS10.0 test statistics processing, analysis the differences