G-CSF动员对供者T淋巴细胞亚群功能及急性移植物抗宿主病影响.docVIP

G-CSF动员对供者T淋巴细胞亚群功能及急性移植物抗宿主病影响 　 作者：刘庆国，杨栋林，黄勇，姜尔烈，周世勇，何依，王志东，王玫 【摘要】 为了研究粒细胞集落刺激因子（G-CSF）动员对供者T淋巴细胞亚群功能和急性移植物抗宿主病（aGVHD）的影响，对20例供者G-CSF动员前后的外周血样品，加入佛波醇酯、伊能霉素、莫能霉素体外刺激培养4小时，以3色荧光标记法流式细胞术检测T细胞亚群Ⅰ（分泌INF-γ、INF-α）、Ⅱ型（分泌IL-4、IL-5和IL-10）细胞比例。结果表明： G-CSF动员前外周血CD3+IFN-γ+ 、CD4+IFN-γ+ 和CD8+IFN-γ+T细胞的比例分别为3.2% (0%-45.9%)、 1.3% (0%-23.8%)和1.5% (0%-22.2%)，G-CSF动员后前述细胞比例显著升高，分别为19.2% (0%-53.9%)、9.5% (0%-49.5%)和7.5% (0%-38.1%)。动员前CD3+IL-2+、CD4+IL-2+、CD8+IL-2+T细胞比例分别为1.5%(0%-31%)、0.8%(0%-30.0%)和0% (0%-5.3%)，动员后比例亦明显升高，分别为25.7% (0%-51%)、19.8% (0%-39.7%)、4.6% (0%-20.9%)。T细胞各亚群的IL-4阳性细胞比例在动员后升高不明显。移植早期aGVHD发生组供者的Tc1细胞比例显著高于无aGVHD组。动员后高Tc2比例组，其受者中、重度aGVHD的发生率明显低于低Tc2比例组（分别为20.0%和77.8%）。结论： G-CSF动员使供者外周血的I型T细胞比例增加，动员后高Tc1细胞比例与移植早期aGVHD发生相关，Tc2比例与中重度aGVHD发生率负相关。 【关键词】 G-CSF动员；T淋巴细胞亚群；移植物抗宿主病 　　Influence of G-CSF Mobilization on Functions of Donor T Lymphocyte Subpopulation and Acute Graft-versus-Host Disease 　　Abstract To investigate the influence of G-CSF mobilization on functions of donor T lymphocyte subpopulation and acute graft-versus-host disease，peripheral blood samples of 20 healthy donors were collected before and after G-CSF mobilization. The whole blood was diluted with IMDM in ratio of 1∶1 and then incubated with PMA +ionomycin+monensin at 37℃，5% CO2 for 4 hours. After being moblized and stained，the IL-4，IFN-γ and IL-2 positive cells were counted with three-color flow cytometry. The results showed that before G-CSF mobilization，the percentages of donor′s CD3+IFN-γ+，CD4+IFN-γ+，CD8+IFN-γ+ T cells were 3.2% (0%-45.9%)，1.3% (0%-23.8%) and 1.5% (0%-22.2%) respectively. The percentage of above mentioned cells in donor increased to 19.2% (0%-53.9%)， 9.5% (0%-49.5%)，7.5% (0%-38.1%) respectively after G-CSF mobilization. The IL-2 positive CD3+，CD4+ and CD8+ T cell percentage in pre-G-CSF mobilized donors was 1.5% (0%-31%)，0.8% (0%-30.0%) and 0% (0%-5.3%) respectively and subsequently increased to 25.7% (0%-51%)，19.8% (0%-39.7%)， 4.6% (0%-20.9%) respectively after G-CSF mobilization.