COX2在慢性白血病骨髓细胞中表达及其意义.docVIP

COX2在慢性白血病骨髓细胞中表达及其意义 　 作者：鲍振华， 李广伦 于军红 论文代写 【摘要】 本 研究 探讨慢性白血病患者骨髓细胞COX2的表达水平及其作用机制和意义。 采用Western blot 方法 检测了67例慢性白血病患者骨髓细胞COX2及内参照βactin的表达水平，以14名健康供者骨髓为阴性对照。 结果表明： CMLCP组COX2的阳性表达率为76.32％(29/38),高于对照组，有显著统计学意义(p=0.0000),CLL组COX2的阳性表达率为75.86％(22/29),高于对照组,有显著统计学意义（p=0.0000）。COX2在CMLCP组和CLL组的表达水平差别无统计学意义（pgt;0.05）。COX2阳性组LDH水平高于COX2阴性组，差别有统计学意义(plt;0.001)。 结论： 在慢性髓系白血病慢性期和慢性淋巴细胞白血病中均表达COX2,而且LDH水平也偏高。 论文代写 【关键词】 慢性白血病 Expression of Cyclooxygenase2 in Bone Marrow Cells of Chronic Leukemia and Its Significance 毕业论文 Abstract The study was aimed to investigate the expression of COX2 in bone marrow cells of chronic leukemia patients and its potential pathogenetic implications. Western blot was applied for detecting COX2 expression levels in bone marrow cells of 67 chronic leukemia patients and βactin expression levels. Bone marrow aspirations from 14 healthy donors were used as negative controls. The results showed that the positive rates of COX2 in chronicphase group of chronic myeloid leukemia (CMLCP) and in group of chronic lymphocytic leukemia (CLL) were 76.32％(29/38) and 75.86％(22/29) respectively. Both CMLCP and CLL group showed a higher expression than control group (p=0.0000,p=0.0000 respectively). The expression of LDH in Cox2 positive group was higher than that in Cox2 negative group, and the difference between the two groups was statistically significant (plt;0.001). It is concluded that the expression of COX2 protEin can be detected in bone marrow cells of CMLCP and CLL and the expression level of LDH were higher in cells of CMLCP and CLL. The expression of COX2 may be correlated with prognosis of CMLCP and CLL. Key words cyclooxygenase2; chronic leukemia; pathogenesis 环加氧酶(cyclooxygenase, COX)是参与花生四烯酸的环氧酶代谢途径中的限速酶,可催化花生四烯酸转化为前列腺素(prostaglandins, PG)。 目前 发现哺乳动物的COX至少有两种异构酶COX1和COX2。COX1是一种结构型酶，是正常细胞的组成蛋白，参与多种生理过程,如保护胃肠道黏膜细胞、调整肾脏血流和控制血小板聚集等。COX2是一种诱导型酶,在正常生理情况下仅少数组织如前列腺、子宫等内有少量表达。但在细胞因子、内皮细胞生长因子、炎症介质、促肿瘤剂以