vWF及HDAC1在胃癌细胞株BGC823中表达及对细胞粘附侵袭迁移能.docVIP

vWF及HDAC1在胃癌细胞株BGC823中表达及对细胞粘附侵袭迁移能 　 作者：王敏，刘伟，杨爱军，王晨昱，李敏 【摘要】 目的：研究血管性血友病因子（vWF）及组蛋白去乙酰化酶1（HDAC1）在人胃癌细胞株BGC823中的表达及对细胞粘附、侵袭、迁移能力的影响，并探讨其机制. 方法：体外培养人胃癌细胞株BGC823，分别以vWF抗体（vWF Ab）及HDAC1抗体(HDAC1 Ab)作用处理；用RTPCR和免疫细胞化学方法检测细胞中vWF和HDAC1的表达及相互作用. 噻唑兰比色（MTT）法检测对细胞外基质成分Ⅳ型胶原粘附能力的变化；Boyden小室法检测细胞侵袭、迁移能力的改变. 结果：BGC823细胞能够表达vWF和HDAC1；经HDAC1 Ab处理后，细胞中vWF mRNA相对含量由处理前的0.2134增加到0.3214，vWF蛋白表达增强；vWF Ab处理组细胞的粘附率较未处理组明显下降（Plt;0.01）；vWF Ab处理组细胞侵袭、迁移穿膜细胞数分别为54.86±9.94和60.47±16.20，与空白对照组相比较差异具有统计学意义（Plt;0.01）. 结论：人胃癌细胞株BGC823能够表达vWF 及HADC1；HDAC1可调节vWF的表达；vWF在 BGC823细胞的粘附、侵袭及迁移过程中起重要促进作用. 【关键词】 胃肿瘤；细胞系，肿瘤；von willebrand因子；组蛋白去乙酰化酶1；肿瘤转移 　　【Abstract】 AIM: To investigate the expressions and effects on adhesion, invasion and motility of von Willebrand factor (vWF) and histone deacetylase 1 (HDAC1) in human gastric cancer cell line BGC823 in vitro and the related mechanism of antimetastasis. METHODS: Human gastric cancer cell line BGC823 cultured in vitro were treated respectively with vWF antibody (vWF Ab) and HDAC1 antibody (HDAC1 Ab). The expressions of vWF and HDAC1 in BGC823 were detected by RTPCR and immunocytochemistry. The effects of vWF and HDAC1 on the adhesion, invasion and motility of human gastric cancer cell line BGC823 in vitro were explored respectively by MTT colorimetric assay and Boyden chamber. RESULTS: The human gastric cancer cell line BGC823 expressed vWF and HDAC1. After treatment with HDAC1 antibody, the expression level of vWF mRNA in BGC823 was increased from about 0.2134 to 0.3214, and the expression of vWF protein was increased. Compared with the control group, the adhesion, invasion (54.86±9.94) and motility (60.47±16.20) abilities of the BGC823 cells were decreased significantly (Plt;0.01) after treatment with vWF antibody. CONCLUSION: Human gastric cancer cell line BGC823 can express vWF and HDAC1, and the expression of vWF can be regulated by HDAC1. vWF takes an important part in the adhesion, invasion and motility of the BGC823 cells. 　　【Ke