β-catenin在白血病细胞系中表达探究.docVIP

β?catenin在白血病细胞系中表达探究 　 ;作者：麦玉洁 ，邱录贵 ，李增军 ，李新， 王国蓉， 毕业论文 ; 于珍，徐燕 ，王亚非 ，李茜 毕业论文 【摘要】; 本研究检测β?catenin在白血病/淋巴瘤细胞系中的表达，探讨其与白血病的关系。采用半定量RT?PCR、免疫细胞化学和Western blot的方法检测β?catenin在白血病细胞系中的表达。结果表明: 白血病细胞系中β?catenin在转录水平上有广泛表达，其中：在U937、KG1a、 Jurkat、K562、Namalwa细胞中存在极高表达；在HEL、HUT78、Raji、Daudi、CEM中有中等表达；而在LCL?H、HL?60、NB4、J6?1、Ramos细胞中β?catenin mRNA呈相对较低水平的表达。蛋白表达水平与mRNA表达水平结果一致。所检测的细胞系中，胞核中均可见β?catenin的表达，但程度不一;在U937、K562、KG1a 和Jurkat细胞的胞核内可见大量分布。结论: β?catenin作为WNT/β?catenin信号转导途径中重要的“调节子”，其在白血病细胞中的过量表达提示WNT/β?catenin信号转导途径可能在白血病细胞中有异常激活。 毕业论文 【关键词】; 白血病细胞系 毕业论文 Expression of β?Catenin in Leukemic Cell Lines 毕业论文 Abstract ; This study was aimed to investigate the expression of β?catenin in leukemic cell lines and its relationship with pathogenesis of leukemia, semi?quantitative RT?PCR and Western blot were performed to detect the expression of β?catenin in a panel of 15 human hematopoietic cell lines (U937, KG1a, Jurkat, K562, Namalwa, HEL, HUT78, Raji, Daudi, CEM, LCL?H, HL?60, NB4, J6?1, Ramos). Immunocytochemistry was; performed in some of these cell lines to detect the location of β?catenin. The results showed that the; β?catenin gene was widely expressed in most leukemic cell lines; in various; degree, the high expression of ; β?catenin was found is U937, KG1a, Jurkat, K562 and Namalwa cells, middle erpression of β?catenin was observed in; HEL, HUT78, Raji, Daudi and CEM cells, lower expression of β?catenin was observed in; LCL?H, HL?60, NB4, J6?1 and; Ramos cells. The expression level of β?catenin protein was identical to the expression level of β?catenin mRNA. The expression of β?catenin could be found in nuclei of all cells mentioned above, but their levels were different belween them.; Abundant β?catenin also could; be observed in nuclei of some leukemic cells by immunocytochemistry.; It is concluded that overexpression of; β?catenin in leukemia cells, as a key mediator of Wnt signaling transduction; pathway, ; indicates that the; Wnt signaling t