三七总皂苷、黄芪对2型糖尿病动脉粥样硬化大鼠修饰型低密度脂蛋.docVIP

三七总皂苷、黄芪对2型糖尿病动脉粥样硬化大鼠修饰型低密度脂蛋 　 作者：李静波，刘宽芝，李金荣 【摘要】 目的 探讨三七总皂苷、黄芪防治2型糖尿病动脉粥样硬化的作用机制。方法 建立2型糖尿病大鼠模型，随机分为糖尿病模型组及糖尿病三七总皂苷、黄芪治疗组，并与正常对照组比较，分别测定各组大鼠血糖、血脂、氧化及糖化低密度脂蛋白水平，并取大鼠主动脉组织进行形态学观察，计算病灶面积与管壁总面积比值。结果 2型糖尿病大鼠血糖、甘油三酯（TG）、总胆固醇（TC）、极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）、氧化低密度脂蛋白（Ox-LDL）和糖化低密度脂蛋白（Gly-LDL）水平升高，高密度脂蛋白（HDL）下降，主动脉病变程度明显。三七总皂苷、黄芪治疗组动物各项指标均有明显改善。结论 三七总皂苷、黄芪可通过降低血糖、调节血脂、抗氧化发挥其防治2型糖尿病AS的作用。 【关键词】 三七总皂苷；黄芪；氧化低密度脂蛋白；糖化低密度脂蛋白；动脉粥样硬化；2型糖尿病 【Abstract】 Objective To investigate the role and mechanism of Astragalus (AS) and Panax Notoginseng Saponins (PNS) in treatment of type 2 diabetic atherosclerosis. Methods Type 2 diabetic rats induced by high-fat diet and a low dose of STZ were divided into two groups randomly: diabetic model rats group treated with AS and PNS as compared with control subjects. Serum level of oxidized low density lipoprotein (Ox-LDL), Glycated low density lipoprotein (Gly-LDL), lipid and blood glucose were measured before and after treatment. Aorta tissue was stained with HE and observed with microscope.Results The serum levels of GLU, TG, TC, LDL-C, VLDL-C, Ox-LDL and Gly-LDL in type 2 diabetic rats were improved, while the levels of HDL-C were decreased. The pathological changes of aorta tissue were obvious in diabetic rats. Each index was improved after treatment with AS and PNS. Conclusion tPNS and Ast can reduce the level of glucose, regulate the serum lipids, antagonize oxidation, thus the two Chinese herbs may prevent and cure diabetic atherosclerosis. 【Key words】 Total panax notoginseng saponins; astragalus membranaceus; oxidized low density lipoprotein; Glycated low density lipoprotein; atherosclerosis; type 2 diabetes 大血管病变是2型糖尿病（DM）的常见并发症，严重威胁着人类的健康，其突出的病理变化是动脉粥样硬化（AS）。近年研究表明，修饰型低密度脂蛋白主要包括氧化低密度脂蛋白（Ox-LDL）和糖化低密度脂蛋白（Gly-LDL）等，在AS的发生、发展过程中起到重要作用。本研究通过高脂膳食喂养SD大鼠，再注射小剂量链脲佐菌素（STZ）诱发胰岛素抵抗和高血糖，从而建立2型糖尿病大鼠模型。在此基础上通过测定大鼠血中修饰型低密度脂蛋白水平探讨其与糖尿病AS的关系。给予糖尿病大鼠三七总皂苷、黄芪干预治疗，探讨两药对糖尿病AS的防治作用及作用机制。 1 材料与方法 1.1 动物 选用雌性SD大鼠，二级动物（购自河北医科大学动物实验中心），共40只，体重180～200g，于清洁动物室饲养