不同剂量配比实验性甲型肝炎戊型肝炎联合疫苗免疫原性探究.docVIP

不同剂量配比实验性甲型肝炎戊型肝炎联合疫苗免疫原性探究 　 【关键词】; 甲型肝炎病毒;,,戊型肝炎病毒;,,联合疫苗;,,剂量配比;,,免疫原性 毕业论文 　　Immunogenicity of experimentally combined hepatitis A and hepatitis E vaccine at different dosage ratio 毕业论文 　　[Abstract]; AIM: To evaluate the immunogenicity of experimentally combined hepatitis A and hepatitis E (cHA+E) vaccine and analyze the interactions between the two antigenic components in vaccine. METHODS: Nine different dosage ratio of the cHA+E vaccine,; A500+E200, A500+E100, A500+E50, A250+E200, A250+E100, A250+E50, A125+E200, A125+E100 and A125+E50 (U+mg)/L,; was prepared from an inactivated hepatitis A (HA) vaccine and a hepatitis E (HE) vaccine. 120 mice were immunized with the cHA+E vaccine,; HA vaccine and HE vaccine alone in different doses,; respectively. Mice were bled before immunization and 2,; 4,; 6,; 8,; 10,; 12 and 14 weeks post immunization. Specific antibodies against hepatitis A and E viruses were detected by ELISA and neutralization assay. RESULTS: After immunization with different dosages of cHA+E vaccine,; all animals developed hepatitis A virus antibody (antiHAV) and hepatitis E virus antibody (antiHEV). AntiHAV responses increased with the concentration of the HA vaccine component in the cHA+E vaccine. AntiHAV neutralizing antibody titers reached 1∶1 024 when mice were immunized with cHA+E vaccine prepared from higher dosage (500 U/mL) of the HA vaccine. The titers only reached 1∶512 when lower dosages (250 and 125 U/mL) of HA vaccine were applied. However,; different dosages (200,; 100 and 50; mg/L) of the HE vaccine component in the cHA+E vaccine developed no effects on the production of antiHAV neutralizing antibody. In addition,; comparing with the single HE vaccine,; the cHA+E vaccine induced higher level of antiHEV response. The antiHEV level increased with the concentration of HA vaccine component in the cHA+E vaccine. However,; there was no obvious relationship between the antiHEV levels and the different dosages （200,; 100 and 50 mg/L）of th