喉鳞癌组织及相邻正常黏膜基因表达谱差异.docVIP

喉鳞癌组织及相邻正常黏膜基因表达谱差异 　【摘要】 目的：研究喉鳞癌组织与相邻正常黏膜的基因表达谱差异。方法：对2例经病理证实的喉鳞状细胞癌标本和邻近的正常黏膜组织标本与基因表达谱芯片进行杂交，利用激光共聚焦荧光检测系统扫描芯片杂交信号，获得样品中基因序列及表达信息。结果：对比2例喉癌和邻近正常组织的基因表达图谱，发现癌组织和正常组织存在差异表达，2例标本重复出现且差异表达的基因共74个，其中喉癌组织表达上调的基因(R5)27个，表达下调的基因(0＜R＜0.2)47个。结论：利用基因表达谱芯片可以在喉鳞状细胞癌组织和邻近的正常组织之间一次比较7000多个基因表达变化，并能找出病理组织中表达异常的基因。 【关键词】 喉肿瘤 鳞状细胞癌 基因表达 基因芯片 ADifferentially expressed genes in laryngeal squamous cell ［ABSTRACT］ Objective: To screen for the differentially expressed genes in laryngeal squamous carcinoma cells and normal laryngeal tissues using cDNA microarray. Methods: The samples from two laryngeal squamous carcinoma tissues were tested using DNA microarrays. The DNAs were fixed on a glass plate by a series of treatments. The mixed probes were then hybridized to DNA microarrays and scanned for fluorescent signals. Differences between the two tissues were produced. Results: Ran belonging to Ras gene family increased 12.207 times, RAB32 belonging to Ras gene family increased 4.904 times and BIRC5 (Survivin) increased 15.495 times. They were significantly highly expressed in laryngeal cancer. The GFI1B gene decreased 0.0265 times. The GATA1 gene was significantly lowly expressed in one laryngeal cancer and was not expressed in carcinoma cells of another patient. Conclusion: cDNA microarray made in our Lab can recognize more than 7000 gene differences between cancer tissues and normal tissues at the same time and find gene variation. These results accord with other studys. ［KEY WORDS］ Laryngeal neoplasms; Squamous cell carcinoma; Gene expression; DNA microarray 喉癌是我国常见的恶性肿瘤之一，其中鳞状细胞癌占90%以上［1］。我们利用基因芯片法，对来源于不同个体、不同细胞周期、不同分化程度的细胞内的mRNA进行检测，分析正常组织与喉癌组织基因表达谱的差异，动态地了解细胞从正常到癌变的不同阶段基因表达谱的变化，为喉癌的早期诊断、治疗及预后评估提供理论基础。 1 资料与方法 1.1 临床标本 2例经病理证实的男性喉癌标本，由天津市第一中心医院耳鼻咽喉-头颈外科提供，患者分别为58、65岁；病理类型均为鳞状细胞癌，1例是T3N0M0，1例T4N1M0。根据临床分期行全喉切除术，术后即刻切取癌组织及邻近正常组织约700?mg，迅速放入液氮冻存。 1.2 表达谱芯片的制备 用自动点样仪(美国PE公司,SpotArray)将大约15?000个基因点于芯片片基上。点样区分为4个DNA矩阵,共包含7267个人类基因, 分为human cancer、 apoptosis、 signal transduction