大孔吸附树脂分离纯化迷迭香中迷迭香酸探究.docVIP

大孔吸附树脂分离纯化迷迭香中迷迭香酸探究 　【摘要】 目的考察大孔吸附树脂对迷迭香酸的吸附性能和纯化效果，寻找从迷迭香中分离纯化迷迭香酸的较优工艺。方法以迷迭香酸的吸附量、解吸率和所得粉末中迷迭香酸的含量为指标，从4种大孔吸附树脂中筛选出较好的D101树脂。通过静态和动态实验，对迷迭香酸在D101树脂上吸附和解吸的条件进行考察和优化。结果采用D101树脂从迷迭香中分离纯化迷迭香酸的较优工艺参数为：上柱液pH 3～4，速度2 BV/h，溶液处理量9 BV，洗脱剂为40%乙醇，洗脱速度1 BV/h，收集洗脱液3 BV。按此工艺条件，迷迭香酸的解吸率为94.8%，3 BV洗脱液浓缩干燥后，所得粉末中迷迭香酸含量为23.7%，树脂经8次重复使用，吸附量和分离效果无明显下降，可以重复使用。结论 D101树脂可从迷迭香中较好的分离纯化迷迭香酸，具有潜在的工业应用价值。 【关键词】 迷迭香酸； 大孔吸附树脂； 迷迭香； 分离； 纯化 ChinaAbstract:ObjectiveTo evaluate the absorption capability and purification effect of rosmarinic acid on macroporous absorption resin, and to find a better method to separate and purify rosmarinic acid from Rosmarinus officinalis L.. MethodsMacroporous resin D101 was more suitable for the separation and purification of rosmarinic acid among four kinds of resins selected with absorption capacity, desorption ratio and the content of rosmarinic acid in the powder obtained as criteria．Optimal conditions for absorption and elution of rosmarinic acid were performed with static and dynamic experiments.ResultsThe result showed that the optimum technology parameters were as follows：the pH value of solution 3 to 4, flow rate 2 BV/h, solution treatment capacity 9 BV, and rosmarinic acid absorbed can be desorbed by 40% alcohol at flow rate 1 BV/h, and collected 3 BV eluent. The desorption ratio of rosmarinic acid was 94.8% according to the absorption and desorption conditions above, and the content of rosmarinic acid in powder obtained from 3 BV eluent through concentration and dry was 23.7%. After eight times of repeated use, the absorption capability and separation effect of the macroporous resin was not decreased obviously, and the macroporous resin could be recycled.ConclusionRosmarinic acid in Rosmarinus officinalis L. can be effectively separated and purified with the macroporous absorption resin D101, and has the potential application value in industry. 　　Key words:Rosmarinic acid；Macroporous absorption resin； Rosmarinus officinalis L.； Separation； Purificatio