大豆小分子肽抗氧化效应初步探究.docVIP

大豆小分子肽抗氧化效应初步探究 　【摘要】 目的通过动物实验来初步探讨大豆小分子肽（分子量300~700Da）的抗氧化作用。方法 采用昆明种小鼠，实验组小鼠分低、中、高［3.0g/（kg·d）、 6.0g/（kg·d）、9.0g/（kg·d ）］三个剂量组灌胃大豆小分子肽25天，测定小鼠耐缺氧能力的影响。老年雄性Wistar大鼠20只，随机分为老年对照组10只，老年实验组10只，老年实验组灌服大豆小分子肽，按6.0g/（kg·d）剂量，老年对照组灌服同体积的生理盐水。第60天末取样测定血红细胞和肝脏中丙二醛含量和超氧化物歧化酶(superoxide dimutase，SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase，GSH-Px)活性。结果 大豆小分子肽实验组中的高剂量组与对照组（生理盐水组）相比（小白鼠死亡只数），耐低压缺氧能力增强，老年实验组与老年对照组比较，血红细胞SOD酶活性升高(Plt;0.05)，肝脏组织SOD酶活性有升高的趋势，但无统计学意义(Pgt;0.05) 。血红细胞与肝脏组织丙二醛含量有减低趋势，但无统计学意义(Pgt;0.05)；GSH-Px酶活性升高(Plt;0.05)。结论 大豆小分子肽（分子量300~700Da）具有一定的提高老龄大鼠体内抗氧化酶活性、抑制脂质过氧化的作用。 【关键词】 大豆小分子肽；抗氧化；耐缺氧能力；SOD 丙二醛；GSH-Px Preliminary study on anti-oxidative capability of soybeanminor peptides 【Abstract】 Objective To study and discuss the anti-oxidationeffectsand the antiaging effectofsoybean minor peptides（MW300-700Da）inold rats．Methods Soybean minor peptides was administered to mice in treated group for 25 days by 3.0g/(kg·d），6.0g/（kg·d），9.0g/（kg·d）respectively，and the changes of the bear anoxia abilitywere measured.Twenty Wister old rats were randomly divided into 2 groups：old control group and testing old group with 10rats in each group．Testingold group were perfusedwith soybean minor peptides6.0g/(kg·d）for 60 days．The rats old control group were fed with water in the same volume．At the end of the 60th days． biochemical index were measured:the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH—Px) and the concentration of malondialdehyde(MDA)in Hb and liver. Results compared with control group，the bear anoxia abilityof the administered mice in treated group were longer，Compared with those in the old control group．The activities of S0D in Hb were decreased(Plt;0.0 5)，the activity of SOD in liver had a increase tendency，but without any significant difference(Pgt;0 05)，and theconcentrations of MDA in Hb and liver had also a increase tendency，but without any significant difference(Pgt;0.05).As compared with those incontrol the the activities of GSH—px had a increa