天然脑活素促细胞增殖及抗细胞衰老作用探究.docVIP

天然脑活素促细胞增殖及抗细胞衰老作用探究 　 作者：李映红 吴正治 吴伟康 李明 张晓丽 陈茵 杨敏 【摘要】 目的：探讨天然脑活素对人胚肺二倍体成纤维细胞（MRC-5）增殖能力和复制性衰老的影响及其机理。方 法： 光镜下观察 MRC-5 细胞衰老表型，常规计数细胞代龄、传代速度， MTT 法检测细胞增殖活度，X-Gal 染色法观察细胞衰老情况，计数衰老细胞百分率。 结果：天然脑活素可维持细胞的非衰老表型，增加 MRC-5 细胞代龄，天然脑活素 孵育 MRC-5 细胞的细胞增殖能力较空白老龄对照组明显升高（P ＜ 0.05），细胞的增殖速度显著加快。另外，天然脑活素连续培养的细胞在老龄阶段衰老细胞数显著低于老龄对照细胞（P ＜ 0.01）。 结论：天然脑活素可升高 MRC-5 细胞增殖能力，有效延缓细胞的复制性衰老。 【关键词】 天然脑活素； 人胚肺成纤维细胞；增殖活度；复制性衰老；延缓 〔Abstract〕 Objective To explore the effects of Natural Cerebrolysin on cell proliferation activity and replicative senescence of human embryonic lung diploid fibroblasts (MRC-5 cells) and its mechanism. Methods To observe the changes of senescent phenotype by light microscope, count the passage ages and velocity, detect the potential of cell proliferation by MTT. The changes of cells senescence of Natural Cerebrolysin-effected MRC-5 cells were studied by X-Gal staining method, and the positivecell rates of senescence cells were detected and analyzed.． Results Natural Cerebrolysin maintainedunaltered cell morphology and increased life span of MRC-5 by at least 18-21 passages. Comparing withthe control senium cells, the proliferation potential of MRC-5 cells incubated with Natural Cerebrolysin was increased obviously (P lt; 0.05), and the passage velocity was enhanced double of that of the controlsenium cells (P lt; 0.01). In addition, the positive cell rate of senescence cells was significantly lower in late passage cells in sequenced multigeneration culture in Natural Cerebrolysin than those of control senium cells (P lt; 0.01), and similar to those of young cells. Conclusion As a result, we concluded that Natural Cerebrolysin could notably promote the proliferation activity of MRC-5 cells and delay the cells replicative senescence efficiently. 〔Key Words〕 Natural Cerebrolysin; Human embryonic lung diploid fibroblasts; Proliferation activity; Delay; Replicative senescence 衰老（senescence）是细胞的重要生命现象之一，绝大多数正常细胞被认为仅有有限的分裂能力，当其经过一定的倍增之后，将进入终末增殖状态，称为细胞衰老。一般来讲，人类胚胎的成纤维细胞在达到衰老时，