大蓟提取液对4种癌细胞生长抑制作用探究.docVIP

大蓟提取液对4种癌细胞生长抑制作用探究 　作者：李煜，王振飞，李瑶，戴宝贞，贾瑞贞，杨利军，黄翔华 【摘要】 　　目的研究大蓟提取液对癌细胞生长的抑制作用。方法细胞形态观察和活细胞计数法研究大蓟提取液对人白血病细胞K562，肝癌细胞HepG2，宫颈癌细胞Hela，胃癌细胞BGC823生长的抑制作用。结果大蓟提取液可使4种癌细胞形态上发生皱缩、变圆、脱壁、碎裂等变化，生长受到明显抑制，抑制率最高可达92.34%。结论大蓟有确切的抑癌作用，并与中医的气血理论、药物归经理论和肝肾同源理论相一致，在体外细胞水平上也有良好体现，对实验室手段研究中医药同样有着重要指导意义。 【关键词】 大蓟 癌细胞 生长 抑制作用 　　Abstract:ObjectiveTo investigate the inhibitory effects of the extract from Cirsium japonicum Fisch.DC the on growth of human carcinoma cells. MethodsMorphological change observation and live cell accounting were used to investigate the inhibitory effects of Cirsium japonicum Fisch.DC on human chronic myloid leukemia K562 cell, hepatoma HepG2 cell, cervical carcinoma Hela cell and gastric carcinoma BGC823 cell. ResultsGrowth of the four kinds of cells was significantly inhibited by the extract from Cirsium japonicum Fisch.DC，the highest inhibitory rate was 92.34% and cell morphological changes included shrinkage, roundness, floating and crumbling. ConclusionCirsium japonicum Fisch.DC has reliable carcinoma-inhibiting effects and the effects conform to the qi and blood theory, the Meridian-reaching theory and the liver-kidney homology theory of traditional Chinese medicine, which shows once again the abundance of basic theories of traditional Chinese medicine which can also be exhibited in vitro and at cell level and have important guidance meaning to Chinese traditional medicine research by laboratory methods. 　　Key words:Cirsium japonicum Fisch.DC; Carcinoma cell; Growth; Inhibitory effects 　　科研与临床紧密结合，是提高科研成效、缩短科研周期、快捷为临床提供理论依据的有效途径。笔者从经中医中药治疗取得显著疗效的癌症病例的处方中筛选抗癌中药，发现大蓟提取液有良好的作用。现将研究情况报告如下。 　　1 器材 　　1.1 药材 　　大蓟生药购于内蒙古中蒙医院药房。取生药适量，六蒸水洗3遍，烘干，称量；用适量无水乙醇浸湿，充分烘干；六蒸水浸泡过夜，武火煮沸，文火煎煮20 min，纱布过滤，滤渣加适量六蒸水置110℃高压蒸气锅内20 min，纱布过滤；合并两次滤液，2℃冰箱中静置72 h，高、中、低速滤纸，0.45 μm，0.22 μm微孔滤膜逐级过滤；滤液蒸发浓缩、灭菌、4℃保存，使用时以培养液稀释至所需质量分数（以生药计算）。 　　1.2 器材与试剂 　　倒置相差显微镜（Nikon eclipseTE2000-U），超净台（苏州净化设备有限公司），CO2培养箱（GALAXY amp; BINDER），24孔培养板（BIOFIL），RPMI1640、高糖DMEM（GIBCO），胎牛血清（TBD）。 　　1.3 癌细胞系 　　人白血病细胞系K