天然脑活素记忆改善效果及其分子机理探究.docVIP

天然脑活素记忆改善效果及其分子机理探究 　作者：吴正治 李映红 李明 陈嫚茵 杨敏 【摘要】 目的：研究中药古方强记汤及天然脑活素对记忆障碍动物模型在记忆改善方面的作用效果；并运用转基因技术进一步验证天然脑活素对学习记忆分子开关 CREB 基因表达的促进作用。方法：将强记汤及天然脑活素分别以 29.3 g/kg和 18.6 g/kg 的量每日经口灌胃给予 SPF 级小鼠，连续 5 d，第 6 天给药 1 h 后制备成记忆障碍模型，第 7 天给药 0.5~1 h 后分 别用跳台法和避暗法测试记忆成绩；另用不同剂量（50 g/mL 和 150 g/mL）天然脑活素水煎液处理大鼠大脑 B104 CNS 神经细胞，以荧光素酶方法检测 CREB 表达水平。结果：强记汤及天然脑活素水煎液对动物记忆障碍模型小鼠记忆成绩中的潜伏时间、错误次数与模型对照组比较均有统计学意义（P lt; 0.01）；而且天然脑活素的改善效果明显优于原方（P lt; 0.01）；不同剂量天然脑活素水煎液 CREB 表达水平与对照组比较有统计学意义（P lt; 0.01），且有剂量反应关系。结论：中药古方强记汤及天然脑活素对实验动物被动回避反应能力的记忆获得、记忆保持及记忆再现均有的改善作用， 能显著增加在 B104 CNS 神经元细胞中 CREB 基因的表达。 【关键词】 天然脑活素；记忆障碍；记忆成绩；CREB；基因表达 〔Abstract〕 Objective To explore the effects of traditional Chinese medicine ancient compound prescription Memory-Enhancing Decoction (MED) and optimized MED on memory improvement of dysmnesia animal, and verify the promotion of optimized MED on the expression of learning and memory molecular switch CREB gene. Methods The SPF mice were treated with MED or optimized MED in dose of 29.3 g/kg and 18.6 g/kg per day respectively by intragastric administration for five days continuously. And then, the various dysmnesia mouse models were prepared respectively with Scopolamine (3 mg/kg), Cycloheximide (120 mg/kg) by intraperitoneal injection and 40% alcohol (0.1 mL/10 g body weight) by intrag astric administration on the sixth day. Next day, memory result was estimated by using Step-down Test and Dark-avoiding Test after the mice were administered for half or an hour. In addition, rat cerebrum central nervous system (CNS) nerves cell line B104 cells were incubated with optimized MED in different dose of 50 g/mL and 150 g/mL for a certain time. Then, we determined the expression of CREB gene by means of luciferase enzyme method. Results Both of MED and optimized MED improved the memory results about latent time and error frequency of dysmnesia mouse models significantly comparing with model control group (P lt; 0.01), and the improvement of optimized MED was more noticeable than tha