小鼠接种HBV外膜小S蛋白产生细胞免疫反应.docVIP

小鼠接种HBV外膜小S蛋白产生细胞免疫反应 　 【摘要】; 目的 研究接种基因重组乙肝病毒疫苗RHBs产生的细胞免疫反应。方法 四组小鼠，腹腔分别接种0、0.65、1.25、2.5、5μg RHBs；4周后分离脾T淋巴细胞，分别检测CTL （cytotoxic T lymphocyte, CTL）及特异性增殖（T lymphocyte proliferation）反应。结果 CTL特异性释放率分别为：42.36%±19.32%、91.21%±22.97%、69.25%±24.13%、51.49%±21.661%，均高于0组小鼠CTL释放率31.21%±9.23% （对照），差异均有显著性（Plt;0.05）；0.65、1.25、2.5、5μg组小鼠特异性增殖刺激指数SI（体外刺激实验组cpm值/体外未刺激对照组cpm值分别为1.61、2.05、3.74、3.62；用精确Fisher方法分析每个小鼠CTL反应与T淋巴细胞特异性增殖反应的相关性，χ2=5.6979, Plt;0.05，明显相关。结论 接种RHBs产生特异性CTL及T淋巴细胞增殖反应。 毕业论文 【关键词】; 基因重组乙肝病毒疫苗；小鼠；接种；CTL；淋巴细胞特异性增殖 毕业论文 【Abstract】; Objective; Aims the induction of hepatitis B virus （HBV） specific cellular immune response was studied in mice immunizated with recombinant HBV vaccine （RHBs）. Methods; BALB/c（H-2d） mice were inoculated intraperitoneally（i.p.）with RHBs at the doses ranging from 0～5μg,4 weeks after the boosting immunization, spleen T lymphocyte were restimulated in vitro with specific peptides or RHBs separately,to detect specific cytotoxic T lymphocyte （CTL） response and specific helper T lymphocyte （HTL） proliferative activity. Results; For CTL response, the percentages of specific release of the spleen lymphocyte in group 0.65、1.25、2.5、5 （mean±standard deviation） were 42.36%±19.32%、91.21%±22.97%、69.25%±24.13%、51.49%±21.661%, which were all greater than that in control group（31.21%±9.23%, Plt;0.05）.The HTL proliferative activity is strongly associated with the CTL response in the mice immunization with RHBs （χ2=5.6979, Plt;0.05 by exact Fisher test）. Conclusion; Optimal HBV specific CTL response and HTL proliferative activity were induced for the mice immunized with RHBs. There were strong association between CTL response and HTL proliferative activity. 毕业论文 ; 【Key words】; gene recombinant HBV vaccine （RHBs）；mice；inoculated ；CTL；lymphocyte spectific proliferation 毕业论文 ; 乙肝病毒（Hepatitis B Virus, HBV）感染是一种危害人类健康的常见病、多发病，全世界HBV感染者有3亿人。接种HBV疫苗产生的特异性体液免疫反应对预防HBV感染有重要意义，而特异性细胞免疫反应在清除体内已有的HBV、治疗HBV感染过程中起着重要作用。 毕业论文 ; 本文给BALB/c小鼠免疫接种不同剂量的HBV外