抗纤灵冲剂对单侧输尿管梗阻及再通后大鼠尿微量蛋白影响.doc

抗纤灵冲剂对单侧输尿管梗阻及再通后大鼠尿微量蛋白影响 　 作者：吴锋 沈丽萍 张新志 黄迪 董飞侠 何立群 【摘要】 　　目的研究单侧输尿管梗阻（UUO）及再通后大鼠尿微量蛋白的变化和抗纤灵对尿微量蛋白变化的影响。方法建立大鼠UUO模型120只和UUO再通模型90只，随机分为正常组、假手术组、模型组、抗纤灵组和科素亚组。于造模后各时间点检测大鼠尿转铁蛋白（TRF）、尿视黄醇结合蛋白（RBP）、尿β2-微球蛋白（β2-M）。结果在UUO模型中，模型组各时间点TRF、RBP、β2-M较正常组和假手术组明显升高（Plt;0.01），并随梗阻时间的延长逐渐升高。在UUO再通模型中，各时间点TRF、RBP、β2-M较正常组和假手术组明显升高（Plt;0.01），并随梗阻时间的延长逐渐降低。与模型组比较，抗纤灵能明显降低两模型中TRF、RBP、β2-M。结论抗纤灵冲剂能减少UUO模型和UUO再通模型中的尿微量蛋白尿，改善两种模型中的肾损伤。 【关键词】 抗纤灵冲剂； 单侧输尿管梗阻； 单侧输尿管梗阻后再通； 尿微量蛋白 　　Abstract：ObjectiveTo explore the effects of “Kang Xian Ling Decoction” (KXL) on urinary microprotein in rats after unilateral ureteral obstruction and recanalization.Methods120 male Sprague-Dawley rats in UUO model and 90 male Sprague-Dawley rats in UUO recanalization model were randomly divided into 5 groups: Normal group, Shame-operated group, Model group, KXL group, and Lostartan group. Urinary transferrin (TRF), retinol-blinding protein (RBP) and β2-microglobuli (β2-M) were tested weekly. ResultsIn UUO model, the levels of TRF,RBP,β2-M in Model group were increased, compared with Normal group and Shame-operated group, and it had significant difference（Plt;0.01）. In UUO model, the levels of TRF,RBP,β2-M in Model group were increased compared with Normal group and Shame-operated group（Plt;0.01）, but they were decreased. Compared with Model group, TRF,RBP,β2-M contents in KXL group were decreased significantly. ConclusionKang Xian Ling Decoction can decrease urinary microprotein in rats after UUO and UUO recanalization, and improve renal fibrosis. 　　Key words：Kang Xian Ling Decoction; Unilateral ureteral obstruction; Recanalization after unilateral ureteral obstruction; Urinary microprotein 　　尿微量蛋白是指尿中超过正常范围但用常规定性或定量的方法难以检出的一些蛋白质。目前检测尿微量蛋白对肾脏疾病的早期诊断具有重要作用，已被认为是诊断早期肾损害的灵敏指标［1］。尿微量蛋白既是肾小球疾病的标志，也是肾间质纤维化病情发展最重要的独立危险因素［2］。 　　肾纤维化是几乎所有慢性肾脏病进展导致肾功能损害的共同病理过程，尤其是肾小管间质的纤维化与慢性肾脏病的预后更密切相关。单侧输尿管梗阻（UUO）是研究肾纤维化的经典实验动物模型。UUO能直接导致肾小管间质纤维化（TIF），并且啮齿类动物的肾纤维化的特征与人类相似［3］。在UUO模型的肾纤维化起始和发展过程中，有许多细胞、分子的变化可以被容易地检测定量，这使得UUO越来越成为一个好