柴胡皂苷d对酒精性肝纤维化大鼠星形细胞活化影响.docVIP

柴胡皂苷d对酒精性肝纤维化大鼠星形细胞活化影响 　 作者：李素婷，杨鹤梅，齐洁敏，梅立新 【摘要】 　　目的研究柴胡皂苷d（SSd）对酒精性肝纤维化大鼠星形细胞（HSC）活化的影响，以探讨SSd抗肝纤维化的作用机制。方法SD大鼠随机分为3组：酒精组、SS-d保护组、正常对照组。利用白酒灌胃辅以高脂饲料方法建立大鼠肝纤维化模型，同时给予SSd保护，12周末处死大鼠。免疫组化检测肝组织α平滑肌肌动蛋白（αSMA）的表达，H.E染色和VanGieson染色观察肝组织病理改变和纤维组织增生，常规生化方法检测肝功能。结果和模型组比较，SSd明显减少肝组织中αSMA的表达；降低血清ALT，AST活性和肝组织羟脯氨酸（Hyp）含量；肝组织病理改变明显好转，纤维组织增生明显减少。结论SSd通过降低αSAM表达，抑制肝HSC的活化达到抗肝纤维化作用。 【关键词】 柴胡皂苷 d 肝纤维化 肝星形细胞 α 平滑肌肌动蛋白 　　Abstract：ObjectiveTo study the effects of saikosaponin-d (SSd) on the activation of Hepatic Stellate Cells(HSC) and to investigate the main mechanism of SS-d in protection of liver fibrosis.MethodsSD rats were randomly divided into 3 groups：normal group,ethanol-fed group, SSd interventional group.56% distilled alcohol and high fat diet were given orally to the rats of the ethanol-fed group and the SS-d group every day,the rats of SSd group were injected intraperitoneally SSd 2.0 mg·kg-1 once daily.Rats were sacrificed 12 weeks later. The expression of αsmooth muscle actin (αSMA) was detected by immunohistochemistry method. Pathological change in liver tissue was observed under light microscope by H.E and Van-Gieson staining; the content of liver tissue hydroxyproline(Hyp) and liver histopathology were also examined by kivchemistry.ResultsCompared with model group，SSd could reduce the expression of αSMA markedly , improve the pathological changes of liver tissue and decrease the production of fibers significantly，decrease the levels of serum alanine transaminase (ALT),aspartate transaminase(AST) and the contents of liver Hyp significantly .ConclusionSSd can inhibit hepatic fibrosis through inhibiting the expression of αSMA in HSC. 　　Key words：SSd； Liver Fibrosis； Hepatic stellate cell； αSMA 　　长期过量饮酒可引起酒精性肝病，包括肝脂肪变及炎症损伤，严重者可发展为肝纤维化，甚至导致难以逆转的肝硬化。酒精性肝纤维化是酒精性肝病发展到肝硬化的重要阶段，是一可逆过程，如能去除不利因素，消除肝纤维化将成功阻断肝硬化的发生［1，2］。研究表明，肝星形细胞（hepatic stellate cell，HSC）是肝纤维化时肝脏细胞中胶原的主要来源细胞，HSC活化是肝纤维化形成的中心环节［3，4］，本实验用白酒灌胃制备大鼠酒精性肝纤维化模型，观察柴胡皂苷d（SSd）对HSC活化的影响，以探讨中药单体SSd对