氨溴索对环丙沙星透过铜绿假单胞菌生物膜影响.docVIP

氨溴索对环丙沙星透过铜绿假单胞菌生物膜影响 　 作者：杨华 余加林 刘官信 陈波曼 毕业论文 【摘要】; 目的 研究氨溴索对环丙沙星透过铜绿假单胞菌生物膜的影响。方法 孔径为0.22μm的医用微孔滤膜建立铜绿假单胞菌生物膜的体外模型；用高效液相色谱(HPLC)方法测定环丙沙星透过生物膜的量，并测定在2和0.75mg/ml氨溴索作用后环丙沙星透过细菌生物膜的量。结果 经过7d的连续培养后，铜绿假单胞菌可在微孔滤膜上形成均匀致密的生物膜。环丙沙星可缓慢透过细菌生物膜，8h左右达到平衡；2和0.75mg/ml氨溴索作用后可以明显增加环丙沙星8h内透过生物膜的量。结论 以医用微孔滤膜为载体可建立铜绿假单胞菌生物被膜体外模型；氨溴索可以显著地促进环丙沙星对细菌生物膜的透过。 毕业论文 【关键词】; 细菌生物膜 环丙沙星 氨溴索 铜绿假单胞菌 渗透限制 毕业论文 ; Effect of ambroxol on permeability of ciprofloxacin across 毕业论文 ; ABSTRACT; Objective; To investigate the effect of ambroxol on the permeability of ciprofloxacin across the bacterial biofilm of Pseudomonas aeruginosa.; Methods; In vitro model of P.aeruginosa biofim using 0.22μm pore diameter medical micropore film was established. The amounts of ciprofloxacin across the bact?erial biofilm was measured by HPLC under different circumstances: physiological saline, 2mg/ml and 0.75mg/ml ambroxol, and clarithromycin was used as positive control.; Results; After continuous seven?day cultivation, P.aeruginosa could form compact and well?distributed biofilm on the surface of micropore film. Ciprofloxacin could slowly permeate through the biofilm and equilibration was achieved in 8h. When combined with ambroxol, the permeability rate of ciprofloxacin significantly increased.; Conclusions; In vitro model of P.aeruginosa biofilm was successfully established using medical micropore film as a supporter, and ambroxol can significantly promote the permeability of ciprofloxacin across the bacterial biofilm of Pseudomonas aeruginosa. 毕业论文 ; KEY WORDS; Bacterial biofilm Ciprofloxacin Ambroxol Pseudomonas aeruginosa Penetration limitation 毕业论文 细菌生物膜(biofilm，BF)是一个具有结构性、协调性和功能性的高度组织群体，BF菌生物学特性与浮游菌显著不同，其环境适应能力更强，可抵抗吞噬细胞作用，逃避宿主免疫，故耐药性极强，使BF相关感染的临床治疗变得非常棘手。在BF细菌的耐药机制中，渗透限制显得尤为重要。由于BF的特殊结构，使抗生素难以渗透， 导致只有BF表面的细菌暴露于有效剂量的抗生素中，而BF深层的细菌难以被杀灭，这也是临床上BF感染难以治愈或迁延反复的原因之一。国外有报道，化痰药N?乙酰半胱氨酸干扰生物膜形成，破坏生BF结构的作用［1］，被认为是一种具有抗菌性质的非抗生素药物。氨溴索(商品名：沐舒坦)是临床治疗中常用的化痰药物之一，与N?乙酰半胱氨酸作用机制上有一定的相似性，但它是否具有破坏BF结构，增加药物渗透