氯胺酮麻醉对犬脑功能影响fMRI显像探究.docVIP

氯胺酮麻醉对犬脑功能影响fMRI显像探究 　【关键词】 氯胺酮；磁共振成像；麻醉；脑 　　fMRI study on effect of ketamine on cerebral function in dogs 　　【Abstract】 AIM: To study the changes of NMR signal intensity in dogs cerebral regions after ketamine application, and to investigate specific brain regions that are sensitive to the anesthetic. METHODS: Stimulation procedure was performed from consciousness, to sedation, to unconsciousness, and finally to analepsia. Scan lasted 60 Dy. Ketamine was injected at a dose of 2 mg/kg with a constant speed infusion pump. Functional magnetic resonance imaging (fMRI) was applied during the entire experiment. A dynamic singleshot EPI sequence was used to perform BOLD functional MR imaging on a 1.5T Philips Gyroscan MR system and the Functool software was also used to analyze these images. RESULTS: For the ketamine anesthesia groups, the decreased signal intensity was observed at sensory cortex (r=0.79, 19.5%), at motor cortex (r=0.81, 16.6%), at thalamus (r=0.66, 4.5%), and at callosal gyrus (r=0.68, 4.7%). The changes of signal intensity were synchonous. However, there was no significant change in other subcortex regions. CONCLUSION: Ketamine anesthesia suppressed such regions as sensory cortex, motor cortex, thalamus and callosal gyrus, which might be the key targets for ketamine action. 　　【Keywords】 ketamine; magnetic resonance imaging; Anesthesia; Brain 　　【摘要】 目的： 应用磁共振成像技术，研究全麻药氯胺酮对犬脑磁共振信号强度变化的影响，探索麻醉药在脑内的可能作用部位. 方法： 刺激程序采用清醒镇静意识消失苏醒的动态过程，扫描为60个动态. 氯胺酮麻醉按2 mg/kg通过采用恒速泵静脉注射，用药方式为输注. 采用1.5T Philips Gyroscan 磁共振系统，运用单次激发EPI扫描序列进行脑功能BOLD法成像并应用Functool软件进行功能分析. 结果： 氯胺酮麻醉可降低感觉皮层、运动皮层的信号强度，其r值分别为0.79, 0.81，信号变化强度分别为19.5%, 16.6%；丘脑和扣带回区信号也受到抑制，r值分别为0.66, 0.68，信号变化强度为4.5%, 4.7%. 上述4个区域信号的抑制变化过程同步；皮层下其他区域包括海马基底节区未引出激活点. 结论： 氯胺酮麻醉抑制感觉皮层、运动皮层、丘脑和扣带回区域信号强度，而这些区域则可能是氯胺酮作用的靶位. 　　【关键词】 氯胺酮；磁共振成像；麻醉；脑 　　0引言 　　fMRI技术是把神经活动和高分辨率磁共振成像技术完美结合，用解剖形态学方法进行功能、代谢显像综合分析技术，它提供了在体研究各种刺激条件下局部脑功能变化的真实情况，因此，能直接在人体探索全麻药的中枢作用部位，并且能够获得更加真实、可靠的信息. 与核医学中PET, SPECT显像技术相比，fMRI不仅具有更好的时间和空间分辨率，而且无创伤，不需要放射活