氟比洛芬酯对大鼠急性痛超前镇痛效果.docVIP

氟比洛芬酯对大鼠急性痛超前镇痛效果 　【摘要】 目的 观察氟比洛芬酯预处理对大鼠急性痛抗伤害效应,探讨氟比洛芬酯超前镇痛效应。 方法 大鼠按Brennan法制成切口疼痛模型,分为氟比洛芬酯组(P组)、对照组(C组),P组于造模前15 min经尾静脉给药,C组于造模后即刻经尾静脉给药。2组分别再分为4组(各8只):即生理盐水组、氟比洛芬酯1,3,9 mg/kg组;分别于术后2,24,48 h进行累积疼痛评分和热板实验,观察注药后反应。 结果 在动物模型建立前或后给药,氟比洛芬酯均能产生量效相关的抗伤害效应,ED50分别为3.85,4.20 mg/kg;95%的置信区间为(3.42,4.28)与(3.96,4.44)。P组3,9 mg/kg的抗伤害效应评分及热痛阈在建立模型后2,24,48 h时低于C组(Plt;0.05)。 结论 氟比洛芬酯能产生量效相关抗伤害效应,具有超前镇痛作用。 【关键词】 氟比洛芬酯; 超前镇痛; 急性痛 ABSTRACT: Objective To observe the preemptive analgesic effect of pretreatment of flurbiprofen axetil on incisional pain in rats. Methods Animal model was made according to the Brennan method. Experimental rats were randomly divided into two main groups: P and C, each containing four subgroups, which were saline, flurbiprofen axetil 1, 3, 9 mg/kg, respectively. Group P received saline or flurbiprofen axetil before incision and Group C 15 minutes after incision through tail vein. Calculative pain scorce test and hot plate test were performed at 2, 24 and 48 h. Results For both group P and C, flurbiprofen axetil(1, 3, 9 mg/kg) produced significant dose-dependent antinociception. The value of ED50was 3.85 and 4.20 mg/kg respectively. Confident intervals were (3.42, 4.28) mg/kg and (3.96, 4.44) mg/kg, respectively. Antinociception score and plantar withdrawl latency of group P were less than group C at 2, 24 and 48 h(Plt;0.05). Conclusion The administration of flurbiprofen axetilthrough tail vein can produce dose-dependent antinociception. Flurbiprofen axetil has showed preemptive analgesic effect. KEY WORDS: flurbiprofen axetil; preemptive analgesia; acute pain 近年来,对超前镇痛的研究和临床应用正方兴未艾［1-2］。氟比洛芬酯是一种以脂微球为载体可供注射的非甾体类抗炎药物(NSAID),因其具有靶向性和减少了NSAID不良反应而备受临床青睐。笔者通过观察氟比洛芬酯预处理对急性痛大鼠累积疼痛评分和热痛阈值变化来评价其抗伤害效应,探讨氟比洛芬酯超前镇痛的可能性及有效性,旨在为临床应用氟比洛芬酯超前镇痛提供实验依据。 　　1 材料与方法 　　1.1 模型制备与分组 　　1.1.1 模型制备 　　64只雄性(200~250 g)SD大鼠［上海斯莱克动物有限责任公司,动物合格证号为SCSK(沪)2003-0003］,1.4%安氟醚麻醉后,按Brennan法［3］从足底近端0.5 cm处向趾部作长约1 cm切口,切开皮肤后挑起足底肌肉并纵向切割,保持肌肉起止点及附着点完整无损。切口按压止血,5-0丝线缝合皮肤,即制成切口疼痛模型。 　　1.1.2 分组