江苏地区不同类型乙型肝炎患者HBV基因分型探究.docVIP

江苏地区不同类型乙型肝炎患者HBV基因分型探究 　【关键词】 肝炎病毒,乙型；基因型；聚合酶链反应 　　HBV genotyping in hepatitis B patients in Jiangsu province 　　【Abstract】 AIM: To investigate the distribution of hepatitis B virus (HBV) genotypes of hepatitis B patients in Jiangsu province and analyze the relationship of HBV genotypes with the serum HBV DNA levels of patients. METHODS: The HBV genotypes and HBV DNA levels of 176 patients in Jiangsu province were detected by realtime quantitative PCR and Taqman MGB probe technology.RESULTS: Among 176 HBVinfected patients, the proportions of genotype C, BC mixedtype, B, A and D were 66.5%(117/176), 22.7%(40/176), 5.1%(9/176), 0.6%(1/176) and 0.6%(1/176), respectively. The results showed there were significant differences between genotype C and genotype BC mixedtype in HBV DNA load(Plt;0.05), and the HBV DNA level of genotype BC mixedtype was significantly higher than that of genotype C. The rate of genotype C among the patients of chronic hepatitis B was 71.4%, higher than that among the patients of acute hepatitis B, liver cirrhosis and hepatocellular carcinoma, but it showed no significant differences in the various clinical types of HBV infection tested(Pgt;0.05). CONCLUSION: Genotype C and genotype BC mixedtype are the two dominant genotypes in the patients in Jiangsu province, and genotype B, A, and D are also found, but not common. The HBV DNA load of genotype B, C mixedtype is significantly higher than that of genotype C. 　　【Keywords】 Hepatitis B Virus; Genotype; polymerase chain reaction 　　【摘要】 目的： 了解江苏地区乙型肝炎病毒基因型分布特征，探讨HBV各基因型与血清HBV DNA水平的关系. 方法：采用荧光定量PCR结合Taqman MGB探针技术，对江苏地区176份乙型肝炎患者血清中的HBV DNA进行基因分型和定量检测. 结果：176例血清中，C型117人（66.5%），B, C型40人（22.7%），B型9人（5.1%），D型1人（0.6%），A型1人(0.6%). C型与B, C混合型在HBV DNA水平上存在统计学差异（Plt;0.05），B, C混合型的HBV DNA水平明显高于C型. C基因型在慢性乙型肝炎患者中的所占比例（71.4%）比在急性乙型肝炎、肝硬化和肝癌患者中所占的比例较高，但差异无统计学意义. 结论： 江苏地区HBV基因型以C型为主, B, C混合型次之，B型较少，D型,A型极少；B, C混合型患者的HBV DNA数量水平高于C型. 　　【关键词】 肝炎病毒，乙型；基因型；聚合酶链反应 　　0引言 　　 　　乙型肝炎病毒(HBV)为一双链DNA病毒，主要引起急、慢性肝炎、肝硬化和肝癌，其基因序