清毒片、养正片对白血病细胞uPA活性影响探究.docVIP

清毒片、养正片对白血病细胞uPA活性影响探究 　 【摘要】 ［目的］观察中药清毒片、养正片对白血病细胞uPA活性的影响。［方法］SD大鼠24只，随机分为4组：空白组用生理盐水，清毒片组用清毒片，养正片组用养正片灌胃,化疗组用阿糖胞苷腹腔注射，观察含药血清对HL60 uPA活性的影响。［结果］与空白对照组相比较，阿糖胞苷和清毒片、养正片药物血清对HL60细胞培养液上清中uPA的活性都有一定程度的影响，而以阿糖胞苷含药血清降低uPA活性的效果较好。［结论］清毒片、养正片的临床 治疗 作用可能与其降低患者体内白血病细胞uPA活性表达水平的作用有关。 毕业论文 【关键词】 清毒片；养正片；白血病；HL60；uPA 　　Abstract： ［Objective］We observed the effect of Qingdupian and Yangzhengpian on the uPA expression in leukemia cells.［Method］248 SD rats wEigh (210±20)g,half male and half female,divided into 4 groups randomly: blank control（0) group,Qingdupian（Q) group，Yangzhengpian（Y) group and Chemotherapy(H) group.Blank control group were fed physiological saline,Q group were fed Qingdupian juice,Y group fed with Yangzhengpian juice,twice a day,3.5 days.H group were injected Arac through peritoneum,once a day,4 times in all.Draw blood of rats 1～2 hours after the last feed,extract the serum,and store the serum at 4℃.Treat the cells with RPM1640 containing 10% every kind of serum for 48 hours,precipitate the cells;the supernatant fluid was collected respectively for ELISA essay to test the activity of uPA.［Result］The laboratory research showed that all the serum had inhibitive effect on uPA activity of the supernatant fluid of HL60 cells,and Arac containing serum had the best effect on declining uPA activity.［Conclusion］Suggest the clinical therapeutic effect of Qingdupian and Yangzhengpian be related to its declining effect on uPA activities of leukemia cells. 　　Key words: Qingdupian;Yangzhengpian;Leukemia;HL60;uPA 毕业论文 　 　1 实验材料 　　1.1 实验材料 清洁级SD大鼠24只（购自广州中医药大学实验动物中心），人早幼粒白血病细胞株HL60（购自中山医科大学实验动物中心），清毒片、养正片生药饮片各1剂(购自广州中医药大学第一附属 医院 ),10%胎牛血清（购自杭州四季青公司），完全RPMI1640培养液(sigma 公司产品)，uPA活性检测ELISA试剂盒（Chemicon International,Inc.产品），计数板，盖玻片，96孔板，加样枪，可处理吸头，一次性注射器，试管，Ep管，玻璃毛细管，灌胃针头，血分析用抗凝管、烧杯等。 　　1.2 实验仪器 倒置显微镜（Olympus产品），细胞培养箱（NAPCO Series 5400 CO2 Incubator），-20℃冰箱（HUALING BCD268WA）、离心机（上海产TDL5型），电热恒温水温箱（上海跃进医疗器械厂），全自动酶标仪(BIORAD Model 3550