灯盏花素对2型糖尿病大鼠肾脏肥大抑制作用及机制探究.docVIP

灯盏花素对2型糖尿病大鼠肾脏肥大抑制作用及机制探究 　【摘要】 　　目的研究灯盏花素(Bre)对2型糖尿病(T2DM)大鼠肾脏肥大的抑制作用与机制。方法建立T2DM大鼠模型，给予灯盏花素腹腔注射治疗，测定大鼠血糖、血脂、总胆固醇、血清胰岛素水平、肾脏肥大指数和单根近端小管(PT)钠泵活性。结果与正常对照组比较，T2DM模型组血糖、血脂、总胆固醇水平都显著升高；胰岛素敏感指数(ISI)显著降低；6周组大鼠PT钠泵活性显著升高，但12周组大鼠PT钠泵活性显著降低；12周组肾脏肥大指数显著升高。与模型对照组比较，Bre治疗组血糖、血脂、总胆固醇水平都显著降低；6周组大鼠PT钠泵活性显著降低，但12周组大鼠PT钠泵活性显著升高；ISI显著升高；12周组肾脏肥大指数显著降低。结论Bre能抑制T2DM大鼠肾脏肥大，其机制可能与降低血糖、血脂和总胆固醇水平，升高ISI和稳定PT钠泵活性有关。 【关键词】 灯盏花素; 2型糖尿病; 肾脏肥大; 钠泵; 大鼠 　　Abstract：ObjectiveTo investigate the effect and mechanism of breviscapine (Bre) on kidney hypertrophy in type 2 diabetic rats.MethodsFemale Wistar rats were developed a rat model of type 2 diabetes mellitus (T2DM). The 6 week group and 12 week group diabetic rats received Bre (10 mg·（kg·d）-1. The following parameters were measured: the blood sugar, blood fat, total cholesterol level, KW/BW, insulin sensitivity index (ISI)，sodium pump activity of PT. Results①Compared with normal control group, blood sugar, blood fat and total cholesterol in the model control group significantly increased, ISI significantly decreased, KW/BW significantly increased in 12w group, sodium pump activity of PT significantly increased in 6w group but significantly decreased in 12w group (Plt;0.01 or Plt;0.05)；②Compared with model control group, blood sugar, blood fat and total cholesterol in the Bre treatment group significantly decreased (Plt;0.01 or Plt;0.05), ISI significantly increased (Plt;0.01)，the KW/BW were significantly decreased in 12w group, sodium pump activity of PT were significant decrease in 6w group but significant increase in 12w group. ConclusionBre can inhibit kidney hypertrophy, it may be caused by the decrease of blood sugar, blood fat, cholesterol and increase of ISI and stabilization of sodium pump activity of PT in T2DM rats. 　　Key words：Breviscapine; Type 2 diabetes mellitus; Kidney hypertrophy; Sodium pump; Rats 　　2型糖尿病(type 2 diabetes mellitus, T2DM)患病率近年来快速增长，寻找到有效、价廉和毒副作用小的中草药用于T2DM的防治具有重要的意义。灯盏花素(breviscapine, Bre)是从灯盏花中分离出的一类黄酮类成分，主要含灯盏