灯盏花素预适应对大鼠心肌缺血再灌注损伤保护作用.docVIP

灯盏花素预适应对大鼠心肌缺血再灌注损伤保护作用 　 作者：王华 郭莉 徐爱丽 孙晖 【摘要】 目的 探讨灯盏花素预适应对大鼠心肌缺血再灌注损伤的保护作用及其可能机制。方法 采用结扎大鼠冠状动脉左前降支，缺血30 min，再灌注120 min的方法建立心肌缺血再灌注损伤(IR)模型,分成假手术组、IR 组、IP 组、灯盏花素预适应组四组造模，再灌120 min后用生理仪记录再灌期左室内压峰值(LVSP)、左室内压上升最大速率(+ dp/dtmax)、左室内压下降最大速率(dp/dtmax)。由颈总动脉抽血检测血清中白介素6(IL6)、肿瘤坏死因子α(TNFα)的含量，观察灯盏花素对二者的影响。结果 与IR组相比，灯盏花素预适应组和IP组的LVSP、±dp/ dtmax明显升高，而IL 6、TNFα的含量降低。灯盏花素预适应组与IP组间比较无统计学意义。结论 灯盏花素对大鼠心肌缺血再灌注损伤有明显的保护作用，可能与预适应样作用有关。 【关键词】 灯盏花素;心肌缺血再灌注损伤;心肌缺血预适应 　　【Abstract】 Objective To investigate the protective effect of Breviscapine preconditioning during myocardial ischemiareperfusion injury in rats.Methods The myocardial ischemic reperfusion model was created with the ligation of left anterior descending coronary artery for 30 minutes and reperfusion persisted for 120 minutes.Fortyeight healthy male SD rats were divided into four groups, false operation group, IR group,IP group and Breviscapine preconditioning group. Through the physiological recorder, LVSP and ± dp/dtmax of the left ventricle were recorded after the reperfusion for 120 minutes. Finally all rats were collected for blood samples from carotid artery taking serum apart for detecting IL6,TNFα to explore the effect of breviscapine on them.Results Compared with whose in IR group, the LVSP and ±dp/ dtmax were higher in IP and breviscapine preconditioning groups. Furthermore, the IL6,TNFα content of IP and breviscapine preconditioning groups reduced obviously. Breviscapine preconditioning group compared with the IP group was not significant in satistics.Conduction Breviscapine had a clear protective effect on myocardial ischemiareperfusion injury,perhaps related to ischemic preconditioning. 　　【Key words】 breviscapine，myocardial ischemiareperfusion，ischemic preconditioning 　　急性心肌梗死是目前心血管疾病中致死率最高的疾病，降低其发生率及死亡率、改善患者生活质量，是目前医学研究的热点，其中研究最多的是心肌缺血再灌注损伤(IR)。寻找有效的药物防治或减轻再灌注损伤，一直是人们关注的课题。而心肌缺血预适应(IP)是已知的心肌最有力的保护机制[1]，用药物代替缺血刺激可产生缺血预适应样保护作用称为药物性预适应[2]。本实验以大鼠为对象建立心肌缺血再灌注损伤模型，通过对血流动力学指标和血清中白细胞介素 6(IL6)、肿瘤坏死