牡丹叶总黄酮抑菌活性探究.doc

牡丹叶总黄酮抑菌活性探究 　 作者：唐浩国，单方方，魏晓霞，李叶，刘严，向进乐，徐宝成 【摘要】 　　目的分别测定了牡丹叶总黄酮提取液、经大孔吸附树脂（D-101、HPD600）和超滤分离后的浓缩液对几种常见微生物的抑菌活性。方法以抑菌圈直径和最低抑菌浓度为指标。结果乙醇提取液对3种细菌的最低抑菌浓度(MIC)分别为：大肠杆菌5.08 mg/ml， 枯草芽胞杆菌2.54 mg/ml，沙门氏菌1.27 mg/ml；HPD600大孔吸附树脂分离浓缩液对3种细菌的MIC值分别为：大肠杆菌4.92 mg/ml，枯草芽胞杆菌2.46 mg/ml和沙门氏菌1.23 mg/ml；D-101大孔吸附树脂分离浓缩液对3种细菌的MIC值分别为：大肠杆菌3.13 mg/ml， 枯草芽胞杆菌3.13 mg/ml和沙门氏菌0.39 mg/ml；超滤工艺分离浓缩液对3种细菌的MIC值分别为：大肠杆菌4.048 mg/ml，枯草芽胞杆菌4.048 mg/ml和沙门氏菌1.012 mg/ml。结论牡丹叶总黄酮对3种细菌均有一定的抑制作用，其中对沙门氏菌的抑制作用最为显著。 【关键词】 牡丹叶 总黄酮 抑菌活性 大孔吸附树脂 超滤 　　Abstract：ObjectiveTo study the antibacterial activity of the extraction solution and concentrated liquid of total flavonoids after separation of the macroporous resin (D-101, HPD600) and ultrafiltration from the leaves of peony. MethodsAntimicrobial circle diameter and minimum inhibitory concentration were used as the indexes. ResultsThe MIC of the ethanol extract to the three kinds of bacteria was as follows: E. coli 4.92 mg / ml, Bacillus subtilis 2.46 mg / ml, Salmonella 1.23 mg / ml; the MIC of the separation of the HPD600 concentrated liquid to the three kinds of bacteria was as follows: E. coli 3.13 mg / ml, Bacillus subtilis 3.13 mg / ml, Salmonella 0.39 mg / ml; The MIC of the separation of the D-101 concentrated liquid to the three kinds of bacteria was as follows: E. coli 4.048 mg / ml, Bacillus subtilis 4.048 mg / ml, Salmonella 1.012 mg / ml; The MIC of the separation of the UF concentrated liquid to the three kinds of bacteria was as follows: E. coli 3.13 mg / ml, Bacillus subtilis 2.46 mg / ml, Salmonella 0.39 mg / ml.ConclusionThe total flavonoids from leaves of peony have a certain effect on the three kinds of bacteria, but the effect of Salmonella is the most notable respectively. 　　Key words：Leaves of peony; Total flavonoids; Antibacterial activity; Macroporous resin; Ultrafiltration 　　早期黄酮粗制品仅仅作为天然黄色素在染料方面应用，20世纪20年代国外把槲皮素与芦丁应用于临床后，逐渐引起了人们的关注。科学家先后研究确定了黄酮类化合物具有抗氧化、清除自由基、消炎、调节血脂、抗癌、抗HIV等广泛的生物活性。随着科研技术的提高和研究的不断深入，黄酮类化合物对微生物的抑制和杀灭作用逐渐引起了人们的重视。 　　近年