维康颗粒预防大鼠疲劳型亚健康实验探究.docVIP

维康颗粒预防大鼠疲劳型亚健康实验探究 　作者：蔡茁,赵晓山，靳文，黄建华，刘艳艳，陈淑娟，孙晓敏，罗仁 【摘要】 目的 探讨维康颗粒对大鼠疲劳型亚健康的预防作用。方法 应用负重强迫游泳＋部分睡眠剥夺复合因素建立疲劳型大鼠模型。将40只大鼠随机分为5组：正常对照，模型对照组，维康颗粒高、中、低剂量组。观察大鼠的行为学表现，记录大鼠首、末次力竭游泳时间，用乳酸试剂盒测定血浆乳酸含量，用自动生化分析仪检测血清尿素氮含量。结果 维康颗粒高、中、低剂量组均能不同程度地改善模型大鼠的行为学表现,提高大鼠力竭游泳时间（P＜0.05），显著降低大鼠力竭游泳后血浆乳酸（P＜0.05）和血清尿素氮水平（P＜0.05）。结论 维康颗粒可以预防疲劳型亚健康的发生。 【关键词】 中药;维康颗粒;预防;亚健康;大鼠 　　Abstract:Objective To observe the effects of Weikanggranules on rat fatigue model of subhealth.Method TThe rat fatigue model of subhealth status was established by partial sleep deprivation in，combinaton with forced swimming load. 40 rats were divided into five groups: the control group, the model group, and three groups administered with Weikanggranules at high, mediate, and low doses respectively. The behavioral changes of rats were observed, and the first and last exhaustive swimming time of rats was recorded. At the end of the experiment, blood was drawn from post abdominal aorta, and blood lactic acid and urea nitrogen levels were determined.Results The behaviors in the groups administered with Weikanggranules at high, mediate, and low doses were remarkably improved; compared with the model group, the last exhaustive swimming time of rats in the gropus administered with Weikanggranules increased significantly （Plt;0.05）, and blood lactic acid and urea nitrogen levels were remarkably reduced （Plt;0.05）.Conclusion Weikanggranules had good effects on prevention of fatiguesubhealth status in rats. 　　Key words:Chinese medicine；Weikanggranules；prevention；subhealth；rats 　　亚健康（subhealth）是指人体处于健康和疾病之间的一种状态。国内进行了较大规模的亚健康状态临床流行病学调研，结果表明亚健康状态的现患率较高（60％～70％），其临床表现复杂，呈多样化、异质性，可分为疲劳型、胃肠型、疼痛型、心理型、社会型等多个亚型，其中疲劳型亚健康居于首位（占一半以上），严重危害了人们的身心健康和工作学习［1-4］。疲劳型亚健康是以疲劳为中心表现的症状群，包括疲劳、乏力、周身不适、精神不振、活力下降等，但是临床上不能确诊为疾病。本课题组根据中医药理论和多年的临床实践基础上研制出防治疲劳型亚健康状态的中药复方——维康颗粒，前期的体外实验表明,维康颗粒可以保护疲劳型亚健康血清对骨骼肌细胞的损伤作用［5］。本研究拟进行中药维康颗粒干预疲劳型亚健康大鼠的在体研究，为其临床应用提供科学依据。 　　1 材料与方法 　　1.1 药品与试剂 　　维康颗粒由黄精、陈皮、党参等中药组成，南方医科大学南方医院提供（生产批，10 g/包。10%水合氯醛（武汉市和昌化工有