绵毛鹿茸草次生代谢产物含量及抑菌活性分析.doc

绵毛鹿茸草次生代谢产物含量及抑菌活性分析 　【摘要】 ［目的］绵毛鹿茸草的次生代谢产物含量与抑菌活性分析。［方法］对绵毛鹿茸草不同提取液的总黄酮、总绿原酸、总生物碱、总皂甙、游离蒽醌等次生代谢产物含量和抑菌活性进行测定。［结果］显示：70%乙醇提取液、60%丙酮提取液对肺炎双球菌、金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌有中等抑制作用，水提取液有弱抑制作用；70%乙醇提取液是肺炎双球菌、金黄色葡萄球菌的良好抑制剂，60%丙酮提取液是大肠杆菌、枯草芽孢杆菌的良好抑制剂。［结论］绵毛鹿茸草提取掖对大肠杆菌的抑菌活性与绿原酸的含量具有显著的正性关，对枯草芽孢杆菌的抑菌活性与蒽醌之间存在极显著的相关性。 【关键词】 绵毛鹿茸草；次生代谢；抑菌活性 　　Abstract：［Objective］To analyse the content of secondary metabolisms and antibacterial activity of Monochasma Savatieri Franch.ex Maxim.［Method］The contents of 5 secondary metabolites such as flavonoid，chlorogenic acid，tannin，phenol and anthraquinones in the different extracts of Monochasma savatieri Franch.ex Maxim were determined.［Results］It showed that the antibacterial activity of the liquids extracted with 70% ethanol，60% acetone on Streptococcus pneuoncae，Staphylococcus aureus，Escherichia coli and Bacillus subtilis were moderate，while that of the liquids extracted with water was weak.The liquids extracted with 70 ethanol were the better inhibitor of Streptococcus pneuoncae and Staphylococcus aureus，while the liquids extracted with 60 acetone were the better inhibitor of Escherichia coli and Bacillus subtilis.［Conclusion］There was positive correlation between the antibacterial activity of the liquids of Monochasma savatieri Franch.ex Maxim on Escherichia coli and the content of the chologenic acid.There was also marked correlation between that of Baillus subtilis and anthra quinones. 　　Key words：monochasma Savatieri Franch.ex Maxim；secondary metabolism；antibacterial activity 　　绵毛鹿茸草 (Monochasma savatieri Franch.ex Maxim)为唇形科鹿茸草属植物，别名白头翁、千重塔、白头毛，分布于浙江、福建、江西，常生于山坡向阳处杂草中或马尾松林下［1］。主要功能为清热解毒，祛风行气，凉血止血，祛痰，用于感冒，慢性气管炎，肺炎，咳血，吐血，便血，牙龈炎，牙髓炎，乳痈，痈肿，小儿口疮，风湿关节痛等。本文对绵毛鹿茸草的不同提取液中总黄酮、总绿原酸、总生物碱、总皂甙及其对肺炎双球菌、金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌抑菌活性进行研究，并探讨这五类次生代谢产物与抑菌活性之间的相互关系。 　　1 实验与方法 　　1.1 实验材料和仪器 绵毛鹿茸草于2007年6月采自浙江千岛湖姥山林场的山坡上。采集时将棉毛鹿茸草全草用湿布包裹，塑料袋封装，立即带回实验室。将材料洗净，防入105℃恒温干燥箱中固定25min，再在80℃条件下干燥24h，研磨后经过0.25mm金属网筛，将烘干样品放入磨口广口瓶，置于干燥器中保存，备用［2］。标准品芦丁、绿原酸、小蘖碱、人参皂甙Rb，1，8二羟基蒽醌购自中国药