肌苷对海人藻酸引起纹状体损伤保护作用.docVIP

肌苷对海人藻酸引起纹状体损伤保护作用 　【关键词】; 肌苷　　Protective effect of inosine on; intrastriatal kianic acid injection induced lesion in the rat　　【Abstract】 AIM: ; To establish the animal model of intrastriatal kainic acid (KA) injection and to investigate the effect of systematic administration of inosine on the striatal lesion size and the number of NADPHd positive neurons in this model. METHODS: KA was stereotaxically injected into the left striatum of rats.; Ten; rats were divided into 2 groups: Inosine group (n=5), receiving ip inosine administration, (50 g#12539;L-1, 75 mg#12539;kg-1) , 3 times each day, from 1 day prior to operation to day 3 postoperatively when the rats were sacrificed, and control group (n=5), receiving normal saline instead of inosine. Nissl stain and NADPHd histochemistry were then performed to investigate the difference of lesion size and NADPHd positive neurons between inosine and saline treated groups.; RESULTS: Inosine decreased the size of; KAinduced striatal; lesion area and increased the number of NADPHd positive neurons. CONCLUSION: Inosine can attenuate KAinduced striatal lesion and show protective effect; on NADPHd positive neurons in the striatum. These findings provide a pontential clinical use of inosine in the CNS disease.　　【Keywords】 kainic acid inosine corpus striatum; NADPHd positive neuron　　【摘要】; 目的：; 建立大鼠纹状体内注射海人藻酸（KA）模型，并观察肌苷对纹状体损伤面积及NADPH脱氢酶（NADPHd）阳性神经元数目的影响.; 方法：; 采用纹状体内注射KA制备模型. 10只大鼠分成2组（n=5），分别于手术前1 d给予肌苷或等量生理盐水（对照组），腹腔注射，每日3次，持续给药至手术后第3日结束，利用尼氏染色和NADPHd的组织化学方法，观察肌苷组和对照组纹状体损伤面积及NADPHd阳性神经元数目的差别.; 结果：; 肌苷可以使KA引起的纹状体损伤面积减小并增加NADPHd阳性神经元的数目.; 结论：; 肌苷可以减轻兴奋性毒素KA引起的纹状体损伤，并对纹状体内的NADPHd阳性神经元具有保护作用.　　【关键词】 红藻氨酸，肌苷，纹状体， NADPHd阳性神经元　　0引言　　肌苷在临床上被广泛地应用于各个领域，传统观点认为在中枢神经系统，肌苷是一种无活性的代谢中间产物，但是最近研究表明肌苷在体外可保护神经元免于损伤. 史明等［1］发现，在用ZnSO4损伤PC12（一种常用的神经元模型）后，单纯给予肌苷即可提高PC12细胞的存活. 由于兴奋性毒素的损伤是各种神经退行性疾患的一个重要的共同机制，因此，我们使用兴奋性毒素海人藻酸（KA）损伤纹状体模型［2］，探讨肌苷是否在体对兴奋性毒性引起的神经细胞损伤具有保护作用.　　1材料和方法　　1.1材料成年SD雄性大鼠10只(第四军医大学实验动物中心提供)，体质量250~300 g. 利用日本Narishiga公司立体定向仪制作